품종별 국산콩 추출물 및 Isoflavone 유도체의 혈소판 응집억제작용

국문 초록 (Abstract)

식생활에서 쉽게 접하고, 다양한 생리활성물질을 함유하고 있는 국산콩에 대하여 품종별로 용매 추출하여 각 추출물에 대한 혈소판 응집에 미치는 영향과 혈소판 응집을 유도하는 collagen과 thrombin에 대한 응집억제작용을 비교하였고, isoflavone 유도체의 함량 및 검정콩 종피 추출물이 혈소판 응집억제에 미치는 효과를 연구하였다. 국산콩 품종별 용매추출물 1 ㎎/mL을 collagen 2 ㎍/mL과 thrombin 0.1 unit/mL으로 유도한 혈소판 응집반응에 메탄올보다 부탄올 추출물에서 강한 응집억제 효과가 있는 것으로 나타났다. 또한 진품콩과 검정콩의 부탄올 추출물이 collagen에 대하여 각각 94%와 95%의 혈소판 응집 억제율이었고 검정콩이 thrombin에서도 95%의 혈소판 응집억제작용을 나타내었다. 혈소판 응집억제율이 가장 강한 진품콩과 검정콩의 isoflavone 함량은 진품콩의 genistein과 daidzein 함량이 검정콩의 genistein과 daidzein의 함량보다 모두 3배가량 높았다. 배당체인 genistin의 함량은 두 품종 모두 비배당체인 genistein과 daidzein 함량보다 높았으며, 총 isoflavone 함량에서도 진품콩이 검정콩보다 높은 함량이었다. Isoflavone유도체의 혈소판 응집억제작용은 collagen으로 유도한 혈소판 응집반응에서 200 ㎍/mL농도에서 genistein>daidzein>genistin 순으로 각각 97%, 38%, 13%로 억제되었고, thrombin으로 유도한 혈소판 응집 반응에서는 약한 억제작용을 나타내었다. 검정콩 종피에서 메탄올 및 부탄올 추출물의 혈소판 응집억제작용은 collagen에서 각각 86%와 61%로 나타내었고, thrombin에서도 36%와 32%의 응집억제작용을 보였다. 이상의 결과에서 진품콩과 검정콩의 혈소판 응집억제작용은 총 isoflavone 함량과 관련이 있으며, isoflavone 유도체에서 -OH group이 3개인 genistein이 -OH group이 2개인 daizein보다 혈소판 응집억제작용이 강하며, 당이 결합된 genistin 배당체의 약한 응집억제작용은 -OH group 수와 배당체의 관련성을 보여주고 있는 것으로 시사되었다.

다국어 초록 (Multilingual Abstract)

Soybean (Glycine max L.) is an increasingly important food source and functional food. Platelet aggregation plays an important role in thrombogenesis and atherosclerosis. Here, we studied the anti-platelet aggregating effects of solvent extracts from Korean soybean varieties and isoflavone derivatives. Nine Korean soybean varieties were extracted by solvents (methanol and buthanol and their extracts was investigated for the inhibition against the aggregation of washed rabbit platelets induced by collagen or thrombin. Maximal inhibition of buthanol extracts against platelet aggregation induced by collagen was 95% in Black-kong and Jinpum-kong. The potency of their inhibition was in the following order: Black>Jinpum>Bokwang>Hwangkum >Pureun>Malli>Danbaek>Danyeob>Jangsu-kong. The Black-kong only seemed to produce the maximal inhi- bition against platelet aggregation induced by thrombin. Total isoflavone content measured was Jinpum-kong (1347.8 ㎍/g) and Black-kong (918.7 ㎍/g). Maximal inhibition of isoflavone derivatives against platelet aggregation induced by collagen was 97% in genistein. The potency of their inhibition was in the following order: genistein>daidzein>genistin. The isoflavone derivatives did not affect the platelet aggregation induced by thrombin. However, Black-kong cortex seemed to produce the optimal inhibition against platelet aggregation induced by collagen. These results suggest that Black-kong and Jinpum-kong may be a good source for antiplatelet agents, and their antiplatelet effect be related to the content and the chemical structure with the number of -OH group and the attached glycoside in the isoflavone derivative.

목차 (Table of Contents)

• Abstract
  
• 서론
  
• 재료 및 방법
  
• 결과 및 고찰
  
• 요약
  
• 감사의 글
  
• 문헌

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