실험적 포도막염에서 pro-inflammatory cytokine의 역할에 관한 연구 = (The) roles of pro-inflammatory cytokines in experimental Uveitis

다국어 초록 (Multilingual Abstract)

Cytokines produced during the effector phases of natural and specific immunity serve to mediate or regulate immune as well as inflammatory responses. In natural immunity, endotoxin directly stimulates mononuclear phagocytes to secrete cytokines which often regulate the synthesis of cytokines from other cells. To evaluate the roles of pro-inflammatory cytokines including TNF, IL-1, IL-6, and IL-8 in experimental uveitis, numbers of infilterating inflammatory cells, IL-6 bioassay, mRNA analysis for various cytokines and IL-8 were investigated. In this study, there was no significant difference in the number of infiltering cells in TNF-R mice compared to congenic controls, but significant difference in the number of infiltering cells was found in IL-1R^-^ and TNF-R^-^ /il-1R^-^ mice. IL-6 level in aqueous humor was reduced in IL-1R^-^ and TNF-R^-^ /il-1R^-^ mice. mRNAs of TNF-α, IL-1α, IL-1Ra, IL-6, and iNOS were detected in all experimental mice after LPS or IL-1α injection. IL-1α and IL-1Rα mRNA were detected in TNF-R^-^ mice without injection of LPS. mRNA expression of IL-1β, IL-10, IFN-γ and CD8 was not detected in all experimental mice with or without LPS injection but IL-10 mRNA was detected in all experimental mice after IL-1α injection. Anti-IL-8 reduced the number of infilterating cells, but the difference was not statisticaly significant. Anti-IL-8 reduced free IL-8 in viterous humor, but total IL-8 level was not reduced with anti-IL-8.
These results suggest that IL-1 is appears to have a more important role than in LPS-induced uveitis, IL-6 may not be indicator of inflammation progress, and IL-8 showes to be minor contributor.

목차 (Table of Contents)

• 목차
• I. 서론 = 1
• II. 실험재료 및 방법 = 4
• III. 결과 = 10
• IV. 고찰 = 31
• V. 결론 = 35
• 참고문헌 = 36
• Abstract = 43