合成 및 天然Gel에 包括固定된 Streptomyces roseochromogens生菌體에 의한 Steriod의 生物學的 變換에 관한 硏究 = A Study on Microbial Transformation by Immobilized Cells of Streptomyces roseochromogenes for 16α-Hydroxylation of Dehydroepiandrosterone

국문 초록 (Abstract)

Streptomyces roseochromogene's 生菌體에 의한 dehydroepiandrosterone으로부터 16α-hydroxylate dehydroepiandrosterone을 生成하는 hydroxylation 反應에 있어서 生菌體를 感光性 架橋劑인 ethylene glycol diacrylate prepolymer (30-G)와 天然高分子物質인 agar gel 및 calcium alginate gel에 각각 包括固定시켜서 反應시켰을 때의 生菌體의 hydroxylase activity의 變化를 比較 硏究하였다. 3種의 gel 모두가 좋은 反應結果를 가져왔다. 그 중에서도 agar gel은 매우 좋을 生菌體의 包括固定에 사용할 수 있는 物質로 思料된다. 그러나 calcium alginate gel은 合成 polymer인 30-G 보다 activity가 낮은 것으로 나타났다.
또한 24時間마다 새로운 反應液으로 交替하며 連續的으로 反應시킨 結果 包括固定된 生菌體의 activity를 매우 좋은 狀態로 오래 持續시킬 수 있었다. 非固定菌體에 比較하여 약 5倍로 延長할 수 있었다.

다국어 초록 (Multilingual Abstract)

The living cells of Streptomyces roseochromogenes were immobilized by entrapment with polyethylene glycol diacrylate (30-G, photo-crosslinkable resin prepolymer), calcium alginate and agar gels, and their transformation ability of dehydroepiandrosterone (DHEA) to 16 α-hydroxylate dehydroepiandrosterone (16 α-OH-DHEA) was studied.
30-G, agar and alginate gels have proved capable of hydroxylation to 16 α - OH-DHEA. Agargels displayer faster rates and higher yields than the both of alginate gels and 30-G gels. The activity of the entrapped cells was maintained fairly longer than that of the (roe cells and the stability of the 16α-hydroxylase activity of the cells over repeated reaction was very much improved by immobilization.

목차 (Table of Contents)

• Ⅰ.序論
• Ⅱ.實驗
• 1.實驗材料 및 實驗方法
• 試藥
• 培養條件
• 2.包括固定
• 3.反應條件
• 4.分析方法
• Ⅲ.結果 및 考察