Transketolase in cultured fibroblasts from Wernicke-Korsakoff patients, Alzheimer's patients and normal controls were compared. The activity and immunoreactivity of transketolase were detected in soluble traction of cultured fibroblasts. The enzyme ac...
Transketolase in cultured fibroblasts from Wernicke-Korsakoff patients, Alzheimer's patients and normal controls were compared. The activity and immunoreactivity of transketolase were detected in soluble traction of cultured fibroblasts. The enzyme activity of the cells from Wernicke-Korsakoff patients was lower(54%) than that of controls, and that of Alzheimer's patients was lower(82%) too. The Western blot assay showed no difference in the electrophoretic mobility of the transketolase protein in among Wernicke-Korsakoff syndrome, Alzheimer's disease, and controls. There were no unique IEF variants in cultured fibroblasts from Wernicke-Korsakoff and Alzheimer's patients.