Isolation and Expression of Red Algae Phycoerythrin on Yeast

다국어 초록 (Multilingual Abstract)

It is increasing necessity of microorganisms for mass production of natural pigments for food application because of both increase of natural pigments demands and liking of natural foods. The phycoerythrins contents of the phycobilisomes of the marine algae of pophyra sp. This work reports the characterization of gene encoding PE in the red alga porphyra sp. The nucleotide sequence of approximately 1.1kb which contain two open reading frames of 565 and 496 nucleotides. These genes were named rpeβ and rpeα. The arrangement of the coding sequences parallels that of the Rodophyta PE genes, with rpeβ and rpeα located 5' of rpeα. The two genes are separated by an intergenic region of 76 nucleotides, and both are transcribed from the same DNA strand. cDNA sequences coding RPE was a member of a homelogous family of peptides, the phycoerythrin. The RT-PCR product of RPE gene was cloned and expressed in yeast.

목차 (Table of Contents)

• Ⅰ. INTRODUCTION
• Ⅱ . Materials and methods
• Materials
• Methods
• Ⅲ. Results
• IV. Discussion