대장암 세포에서 Axl의 GSK3β 신호체계를 통한 EpCAM의 조절기작 = Regulatory mechanism of Axl-mediated EpCAM expression through GSK3-β signaling in colon carcinoma cells

목차 (Table of Contents)

• CONTENTS ⅰ
• ABSTRACT 1
• I. INTRODUCTION 5
• II. MATERIALS AND METHODS 18
• 1. Mice 18
• 2. Cell lines, antibodies and reagents 18
• 3. Isolation of primary colon cells 19
• 4. Small interfering RNA (siRNA) 19
• 5. Isolation of total cellular RNA 20
• 6. Reverse transcription-polymerase chain reaction (RT-PCR) 20
• 7. Western blot analysis 21
• 8. Isolation of nuclear extract 22
• 9. Electrophoretic Mobility Shift Assay (EMSA) 23
• 10. Flow cytometric analysis 23
• 11. Cell proliferation assay 24
• 12. Cell cycle analysis and measurement of apoptosis using flow cytometer 24
• 13. Wound-Healing Assay 24
• Ⅲ. RESULTS 25
• 1. Upregulation of EpCAM expression by Axl in colon carcinoma cells 26
• 2. Regulation of EpCAM expression through Axl-mediated AKT/GSK3β signaling pathway 29
• 3. Transcriptional activation of AP-1 through Axl-mediated AKT/GSK3β signaling pathway 31
• 4. Expression of EpCAM in the colon cells isolated from wild type and Axl-/- mice 31
• 5. Inhibition of Axl-stimulated colon carcinoma cell proliferation by anti-EpCAM 34
• 6. Effect of anti-EpCAM on Axl-mediated cell cycle in colon carcinoma cells 34
• 7. Inhibition of MC38 cell migration by blockade of EpCAM 37
• Ⅳ. DISCUSSION 40
• ABSTRACT (IN　KOREAN) 45
• Ⅴ. REFERENCES 48