鉤蟲感染初期의 鐵分代謝에 關한 實驗的 硏究 = Studies on Iron Metabolism in The Early Stage of Hookworm Infection

다국어 초록 (Multilingual Abstract)

There have been several reports concerning iron metabolism in hookworm infection of adult stage of hookworm. Masuya (1960), Nagayama (1960) reported that there was no evidence to show a disturbance of mobilization or utilization of iron in hookworm disease.
Kitayama(1950) presumed that anemia-inducing factor of hookworm would exist through-out the life history of hookworm, regardless the egg or adult stage. Recently, Lee (1965) reported that any anemia-inducing toxin contained in the larval metabolite or larval body was believed to have no connection with the factor of early stage anemia of hookworm, infection. Therefore the anemia- inducing factors in the early stage of hookworm disease were believed to be the blood loss due to the lung damage and early blood sucking in the intestine. The present study is designed to confirm whether the iron metabolism is related to the early stage of hookworm. anemia.
1. Two groups of dogs were infected with 5,000 and 10,000 hookworm larvae respectively and the following research has been carried out.
a) Hematological examination: Five digs were infected with 10, 000 hookworm larvae each and two dogs with 5, 000 larvae each. Hemoglobin(by Cyanmethemoglobin method) was measured before and until 10 days after infection. Hemoglobin was decreased 2 days after infection. However, the Sideroblasts in the bone marrow showed no decrease (15~17) at the very early stage, but at the end of a month it decreased remarkably (1~4 % ).
b) Serum iron: Four dogs were infected with 10,000 larvae and two dogs with 5, 000 larvae. The titration was carried on with junior electric photometer using 2,2'-Dipyridyl.The infected groups showed a decrease of serum iron at 2~5 days after infection but recovered in 10 days after the infection.
c) Iron absorption: 10, 000 larvae and 5, 000 larvae were given to two dogs of each group. Ferric alum (3mg/kg) and Ascorbic acid (200mg) were given orally to five dogs including one control and the titration were carried out every 2 hours for 8 hours with Junior electric photometer using 2,2'-Dipyridyl. Generally it showed a peak during 2~4 hours after the administration and the rate of absorption increased in 5, 000 or 10 000 larvae group and the control group.
d) Postrnortem examination: Five dogs were sacrificed 4.5-and 7 days after infection. A piece of middle portion of small intestine, bone marrow (Ilium), liver, spleen and lung were sliced, fixed and stained in routine way. There were no special changes except the inflammatory process and hemorrhage in the lungs, and the proliferated bone marrow.