Genistein과 Daidzein이 대장암 HCT-116 세포의 성장에 미치는 영향 = Effects of Genistein and Daidzein on the Growth of HumanColon Cancer HCT-116 Cells

다국어 초록 (Multilingual Abstract)

Background : Genistein and daidzein are two major soybean isoflavones. They have received increasing attention because of their possible roles for cancer prevention. However, their mechanisms of action and molecular targets on the human colon cancer cells are not fully understood. Methods : Human colon cancer HCT-116 cells were treated with genistein and daidzein to investigate their effects on the cell growth and this was analyzed with MTT assay. TUNEL assay and Hoechst33342 stain were carried out to identify apotosis. Results : Daidzein was able to inhibit cell proliferation and induce apoptosis of the HCT-116 cells, but genistein didn't affect the cell growth. The ER antagonist ICI182780 didn’t attenuate the antiproliferative and proapoptotic effects of daidzein: this means the effect of daidzein on the HCT-116 cells may not be dependent on the ER pathway. The other soybean isoflavone, genistein, attenuated the effects of daidzein on the HCT-116 cells and its mechanism should be elucidated. Conclusions : These data suggest that daidzein may act as a preventive agent on human colon cancer, and its mechanism of action doesn’t involve the ER-dependent pathway.

참고문헌 (Reference)

1 "Use of azoxymethane-induced foci of aberrant crypts in rat colon to identify potential cancer chemopreventive agents" 15 : 1049-54, 1994

2 "The natural tyrosine kinase inhibitor genistein produces cell cycle arrest and apoptosis in Jurkat T-leukemia cells" 18 : 431-9, 1994

3 "Soyfoods,isoflavones and risk of colonic cancer:a review of the in vitro and in vivo data" 12 : 707-728, 1998

4 "Soybean isoflavones reduce experimental metastasis in mice" 129 : 1075-1078, 1999

5 "Soy of dietary source plays a preventive role against the pathogenesis of prostatitis in rats" 43 : 557-64, 1992

6 "Prevention of precancerous colonic lesions rats by soy flakes,soy flour,Genistein and calcium" 29-,

7 "Prevention of metastatic pancreatic cancer growth in vivo by induction of apoptosis with genistein,a naturally occurring isoflavonoid" 26 : 264-273, 2003

8 "Phytoestrogens and breast cancer" 83 : 113-118, 2002

9 "Phytoestrogen content of foods -- a compendium of literature values" 26 : 123-148, 1996

10 "Phyto-oestrogens,their mechanism of action:current evidence for a role in breast and prostate cancer" 91 : 513-531, 2004

11 "Phyto-oestrogens and cancer" 3 : 364-373, 2002

12 "Phyto-oestrogens" 17 : 253-271, 2003

13 "Nonsteroidal anti-inflammatory drug-activated gene(NAG-1)is induced by genistein through the expression of p53 in colorectal cancer cells" 105 : 747-753, 2003

14 "Inhibitory effect of genistein and daidzein on ovarian cancer cell growth" 24 : 795-800, 2004

15 "Inhibition of proliferation and induction of apoptosis by genistein in colon cancer HT-29 cells" 215 : 159-166, 2004

16 "Genisteininduced G2-M arrest,p21WAF1 upregulation,and apoptosis in a non-small-cell lung cancer cell line" 31 : 184-1891, 1998

17 "Genistein-induced cell cycle arrest and apoptosis in a head and neck squamous cell carcinoma cell line" 34 : 12-19, 1999

18 "Genistein induces apoptosis and topoisomerase IImediated DNA breakage in colon cancer cells" 36 : 796-802, 2000

19 "Genistein induced upregulation of p21WAF1,downregulation of cyclin B,and induction of apoptosis in prostate cancer cells" 32 : 123-131, 1998

20 "Effects of soybean extract on morphology and survival of Caco-2,SW620,and HT-29 cells" 42 : 131-140, 2002

21 "Effects of a soybean isoflavone mixture on carcinogenesis in prostate and seminal vesicles of F344 rats" 90 : 393-398, 1999

22 "Dietary habits and gastro-intestinal cancers:a comparative case-control study of stomach and large intestinal cancers in Nagoya,Japan" 76 : 705-16, 1985

23 "Dietary factors in colon cancer:international relationships" 6 : 160-70, 1984

24 "Dietary Isoflavones:biological effects and relevance to human health" 129 : 758S-767S, 1999

25 "Biphasic effect of daidzein on cell growth of human colon cancer cells" 42 : 1641-1646, 2004

26 "Antiproliferative effects of isoflavones on human cancer cell lines established from the gastrointestinal tract" 53 : 5815-21, 1993