Ribosomal Intergenic Spacer 1 Based Characterization of Button Mushroom (Agaricus bisporus) Strains = Ribosomal Intergenic Spacer 1 Based Characterization of Button Mushroom (Agaricus bisporus) Strains

다국어 초록 (Multilingual Abstract)

Breeding the button mushroom requires genetic information about its strains. This study was undertaken to genetically characterize four domestically bred button mushroom strains (Saea, Saejung, Saedo, Saeyeon cultivars) and to assess the possibility of using the intergenic spacer 1 (IGS1) region of rDNA as a genetically variable region in the genetic characterization. For the experiment, 34 strains of Agaricus bisporus, two strains of A. bitorquis, and one strain of A. silvaticus, from 17 countries were used. Nucleotide sequence analysis of IGS1 rDNA in these 37 Agaricus strains confirmed that genetic variations exist, not only among the four domestic strains, but also between the four domestic strains and foreign strains. Crossing two different haploid strains of A. bisporus seems to generate genetic variation in the IGS1 region in their off-spring haploid strains. Phylogenetic analysis based on the IGS1 sequence revealed all A. bisporus strains could be differentiated from A. silvaticus and A. bitorquis strains. Five genetic groups were resolved among A. bisporus strains. Saejung and Saeyeon cultivars formed a separate genetic group. Our results suggest that IGS1 could be complementarily applied in the polymorphism analysis of button mushroom.

참고문헌 (Reference)

1 Schmidt O, "Ribosomal DNA intergenic spacer of indoor wood-decay fungi" 62 : 759-764, 2008

2 Kim SH, "Rapid detection of Ophiostoma piceae and O. quercus in stained wood by PCR" 65 : 287-290, 1999

3 Bunyard BA, "Phylogeny of the genus Agaricus inferred from restriction analysis of enzymatically amplified ribosomal DNA" 20 : 243-253, 1996

4 민경진, "PCR 다형성에 의한 양송이(Agaricus bisporus) 계통의 유전적 다양성 분석" 한국균학회 42 (42): 1-8, 2014

5 Bonuso E, "Multilocus phylogenetic and coalescent analyses identify two cryptic species in the Italian bianchetto truffle, Tuber borchii Vittad" 11 : 1453-1466, 2010

6 Kerrigan RW, "Meiotic behavior and linkage relationships in the secondarily homothallic fungus Agaricus bisporus" 133 : 225-236, 1993

7 Tamura K, "MEGA6: molecular evolutionary genetics analysis version 6.0" 30 : 2725-2729, 2013

8 Hyuk Woo Kwon, "Genetic and Biochemical Characterization of Monokaryotic Progeny Strains of Button Mushroom (Agaricus bisporus)" 한국균학회 43 (43): 81-86, 2015

9 Raper CA, "Genetic analysis of the life cycle of Agaricus bisporus" 64 : 1088-1117, 1972

10 Saito T, "Characterization of subrepeat regions within rDNA intergenic spacers of the edible basidiomycete Lentinula edodes" 66 : 2125-2133, 2002

11 Thompson JD, "CLUSTAL W : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice" 22 : 4673-4680, 1994

12 Fritsche G, "Breeding mushrooms" 157 : 4-17, 1986

13 Bertoldo C, "Assessment of genetic variability of some strains of Fusarium spp. isolated from Lisianthus by analysis of TEF sequences, IGS and RAPD" 2 : 74-, 2011

14 Huang CY, "Analysis of intergenic spacer 2 diversity of ribosome DNA for strains of Pleurotus eryngii" 13 : 592-595, 2005

15 Foulone-Oriol M, "Agaricus bisporus cultivars: hidden diversity beyond apparent uniformity?" 9-16, 2011