최우수논문상(생식내분비학) = Comparison of estrogen receptor-a, progesterone receptor and calponin expression in gonadotrophin-releasing hormone agonist-sensitive and -resistant uterine fibroids

다국어 초록 (Multilingual Abstract)

Objective: This study was aimed to compare immunohistochemical expression of estrogen receptor (ER)a, progesterone receptOf (PR), and calponin in gonadotrophin-releasing horrrone agonist (GnRH-a)-sensilive and -resistant uterine fibroids. Methods: We collected data retrospectively. The sensitive group consisted of women who had reduction in uterine volume greater than 40% following GnRH-a treatment. Uterine volume was either reduced by less than 10%, or was increased in the resistant group. A tissue microarray was constructed using formalin-fixed, paraffin-embedded tissues, 31 and 26 patients for the sensitive and resistant groups, respectively. Tissue sections were immunostained with antibodies against ER-a, PR. and calponin. The intensity and area of the immunohistochemical reactions were evaluated using a semi-quantitative scoring system. The Mann-WhitneyU-test, Fisher``s exact test. and Spearman``s rank correlation test were used for analysis of data. Results: PR (P= 0.04) and calponin (p= 0.03) showed a significantly higher staining intensity in the resistant group than in the sensitive group. Both groups showed comparable expression of ER-a (P = 0.23). In correlation analysis between changes in uterine volume after GnRH-a therapy and clinicopathological factors, the immunohistochemical intensity 01 PR (P= 0.04) 2nd calponin (P= 0.03) was significantly correlated with changes in uterine volume. Conclusion: This study shows that GnRH-a resistance of uterine fibroids is not related to ER-a content. but the expression of PR and calponin is related with GnRH-a resistance.