Aspergillus niger에 의한 글루콘산의 생산 = (The) production of gluconic acid by Aspergillus niger

다국어 초록 (Multilingual Abstract)

The production of gluconic acid from glucose by the fermentation of Aspergillus niger(KCTC 2119) was studied.
It was found that the conversion products from glucose by filamentous fungi were markedly influenced by the pH, temperature, substrate concentration, inoculum size and medium composition.
Cells of A. niger were grown well on the medium containing 40g/L of glucose, whereas the growth was inhibited with the glucose concentration at higher than 40 g/L.
The higher value of dry cell weight and productivity were obtained when adding 0.1 g/L of corn steep liquor.
Optimum concentration of (NH₄)₂SO₄for gluconic acid production was 0.5 g/L.
Optimum concentration of MgSO₄ㆍ7H₂O used as a sulfur source was 1 g/L. It was also found that the cell concentration and gluconic acid production were at the maximum level when grown on the medium containing 0.2g/L KH₂PO₁, and 0.2g/L K₂HPO₄. Also, the optimum culture conditions were 33℃ and pH 5.5 and the maximum gluconic acid production was obtained in the culture medium 110 g/L glucose, 0.5 g/L (NH₄)₂SO₄ at 33℃ and pH 5.5.
The metabolic parameters such as specific growth rate, cell and gluconic acid yields and overall gluconic acid productivity were estimated for process improvement.

목차 (Table of Contents)

• 목차
• List of Tables = iii
• List of Figures = iv
• ABSTRACT = v
• I. 서론 = 1
• II. 이론적 고찰 및 연구배경 = 4
• II-1. 미생물 균주 배양에 영향을 미치는 인자 = 4
• II-2. Gluconic acid의 물성 = 11
• II-3. Gluconic acid의 용도 = 12
• II-4. Gluconic acid의 생합성 = 14
• II-5. 글루콘산의 생성에 관여하는 A.niger의 효소들 = 16
• II-5-1. Glucose Oxidase = 16
• II-5-2. Catalase = 17
• II-6. 사상균의 증식 = 20
• III. 재료 및 방법 = 23
• III-1. 사용균주 = 23
• III-2. 균주의 보존 = 23
• III-3. 배양액 = 24
• III-4. 배양 및 발효조건 = 26
• III-5. 분석방법 = 26
• IV. 결과 및 고찰 = 29
• IV-1. 성장곡선 = 29
• IV-2. 접종량의 영향 = 29
• IV-3. 탄소원의 영향 = 32
• IV-4. Glucose 농도의 영향 = 32
• IV-5. 초기 pH의 영향 = 35
• IV-6. 질소원의 영향 = 35
• IV-7. 온도에 따른 영향 = 39
• IV-8. 2.5L jar fermentor에서의 회분식 배양 = 39
• V. 결론 = 45
• 참고문헌 = 47