Characterization of Aroma Extracts Isolated from Medicinal Plants : 약용식물체에서 분리한 향기 성분의 분석과 항산화 능에 관한 연구

국문 초록 (Abstract)

건조된 감국, 고본 (稿本), 독활, 박하 (薄荷), 석창포, 송엽 (松葉), 우슬, 정향 (丁香), 창이자 (蒼耳子), 총백 (蔥白), 형개 (荊芥), 홍화 (紅花), 흑두 (黑豆)는 독특한 향을 지닌 한국의 전통적인 약용 식물체이다. 그들이 지닌 향기 성분의 항산화 능에 대하여 연구하였다. 약용 식물체의 향기 성분은 감압하의 증기 증류와 dichloromethane을 용매로한 액체-액체 연속 추출을 이용하여 추출하였다. 추출된 향기 성분은 gas chromatography/mass spectrometry (GC/MS)와 gas chromatography-flame ionization detector (GC-FID)를 이용하여 정성 및 정량 분석하였다. 향기 추출물의 항산화 능은 aldehyde/carboxylic acid assay를 사용하여 평가되었다. 200 ㎍/mL 수준에서, 30일 동안 고본, 독활, 박하, 석창포, 형개, 홍화의 향기 추출물의 항산화 능은 홍화 > 석창포 > 고본 > 박하 > 독활 > 형개 순이었다. 향기 추출물의 항산화 능은 잘 알려져 있는 항산화제인 α-tocopherol (Vitamin E)와 butylated hydroxytoluene (BHT)와 비교하였다.

다국어 초록 (Multilingual Abstract)

Dried Chrysanthemi Flos (Chrysanthemum morifolium Ramatuelle var. sinense Makino), Angelicae tenuissimae Radix (Angelica tenuissima Nakai), Araliae Radix (Aralia continentalis Kitagawa), Menthae Herba (Mentha arevensis L.), Acori Rhizoma (Acorus gramineus Soland), pine needles (Pinus sylvestris L.), Achyranthis Radix (Achyranthes japonica (Miquel) Nakai), clove buds (Syzygium aromaticum (L.) Merr. et Perry), Xanthii Fructus (Xanthium strumarium L.), Allium (Allium fistulosum L.), Nepetae Herba (Schzonepeta tenuifolia Briq.), Carthami Flos (Carthamus tinctorius L.), and black beans (Phaseolus vulgaris L.) are traditional medicinal plants in Korea and have their own characteristic aromas. Aroma compounds isolated from the medicinal plants were obtained using liquid-liquid continuous extraction following steam distillation under reduced pressure (55℃ and 95 mmHg). Aroma compounds were identified in the extracts using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Antioxidant properties of the aroma extracts were investigated by aldehyde/carboxylic acid assay. At a concentration of 200 ㎍/mL, the antioxidative activities in the aroma extracts of Angelicae tenuissimae Radix, Araliae Radix, Menthae Herba, Acori Rhizoma, Nepetae Herba and Carthami Flos were in the following descending order: Carthami Flos > Acori Rhizoma > Angelicae tenuissimae Radix > Menthae Herba > Araliae Radix > Nepetae Herba, for 30 days. The antioxidative activities in aroma extracts were comparable to that of the known antioxidants, α-tocopherol (Vitamin E), and butylated hydroxytoluene (BHT).

목차 (Table of Contents)

• CONTENTS
• ABSTRACT
• Ⅰ. Introduction = 1
• Ⅱ. Materials and Methods = 5
• 1. Materials = 5
• (1) Materials = 5
• (2) Reagents = 5
• 2. Methods = 6
• (1) Isolation of Aroma compounds = 6
• (2) Identification of Aroma Compounds Isolated from Extracts = 7
• (3) Determination of Total Aroma Compounds in Extracts = 7
• (4) Aldehyde/Carboxylic Acid Assay = 8
• (5) Instrumental Analysis = 9
• Ⅲ. Results and Discussion = 10
• 1. Identification of the Aroma Compounds Isolated from the Medicinal Plants = 10
• (1) Chrysanthemi Flos (Chrysanthemum indicum L.) = 10
• (2) Angelicae tenuissimae Radix (Angelica tenuissima Nakai) = 15
• (3) Araliae Radix (Aralia continentalis Kitagawa) = 18
• (4) Menthae Herba (Mentha arevensis L.) = 22
• (5) Acori Rhizoma (Acorus gramineus Soland) = 26
• (6) Pine neddles (Pinus sylvestris L.) = 29
• (7) Achyranthis Radix (Achyranthes japonica (Miquel) Nakai) = 33
• (8) Clove buds (syzygium aromaticum (L.) Merr. et Perry.) = 36
• (9) Xanthii Fructus (Xanthium strumarium L.) = 39
• (10) Allium (Allium fistulosum L.) = 43
• (11) Nepetae Herba (Schzonepeta tenuifolia Briq.) = 47
• (12) Carthami Flos (Carthamus tinctorius L.) = 51
• (13) Black beans (Phaseolus vulgaris L.) = 54
• 2. Antioxidant Activity of Aroma Extracts Isolated from Various Medicinal Plants Measured by the Aldehyde/Carboxylic Acid Assay = 57
• (1) Antioxidant Activity of Known Antioxidants (a-tocopherol & BHT) = 58
• (2) Antioxidant Activity of Extracts Isolated from Medicinal Plants = 61
• (3) Comparable to that of Antioxidant Activity of Extracts = 70
• Ⅳ. Conclusion = 74
• 참고문헌 = 75
• 국문요약 = 83