Enhancement Effect of Cell Adhesion on Titanium Surface Using Phosphonated Low-Molecular-Weight Chitosan Derivative

다국어 초록 (Multilingual Abstract)

For improving cell adhesion on titanium surface, phosphonated low-molecular-weight chitosan (P-LMC) was prepared by introducing a phosphonate group of 4-phosphonobutyric acid to low-molecular-weight chitosan (3,000<MW<10,000). The phosphonate group of P-LMC was identified by 31P NMR. At about 30 ppm from external standard, there was a specific peak derived from phosphonate group. X-Ray Photoelectron Spectroscopy (XPS) and water contact angle were carried out for characterization of titanium surface treated with P-LMC. From these results, P-LMC coated on titanium surface was remained on that in spite of washing and P-LMC made titanium surface more hydrophilic. The biocompatibility of P-LMC was demonstrated through a cytotoxicity test using the C2C12 cell line because P-LMC should have no cytotoxicity for applying to dental implant materials. As a result, P-LMC was biocompatible to use as coating material of titanium. Improvement of cell adhesion on titanium surface treated with P-LMC was observed by microscopy, and the number of attached cells was counted using a hemocytometer.
Titanium surface coated with P-LMC showed improved cell adhesion for 12 h. These results demonstrate that P-LMC is biocompatible and titanium surface treated with P-LMC has positive effects on cell adhesion. Therefore, P-LMC is a useful material that can be applied for titanium coating to enhance cell adhesion and osseointegration.

참고문헌 (Reference)

1 S. Hayakawa, 25 : 375-, 2014

2 S. -H. Park, 63 : 1616-, 2014

3 E. Mohseni, 48 : 238-, 2014

4 X. Liu, 47 : 49-, 2004

5 K. -I. Kim, 117 : 3029-, 2010

6 M. Rinaudo, 31 : 603-, 2006

7 M. Sadeghi-Kiakhani, 1 : 406-, 2013

8 Y. -M. Cho, 129 : 2161-, 2013

9 H. Zitter, 21 : 881-, 1987

10 R. Strietzel, 19 : 1495-, 1998

11 B. Kasemo, 13 : 8-, 1999

12 R. Fraioli, 128 : 191-, 2015

13 H. Oonishi, 71 (71): 213-, 1989

14 H. -M. Kim, 32 : 409-, 1996

15 L. Le Guéhennec, 23 : 844-, 2007

16 P. R. Klokkevold, 8 : 442-, 1997

17 X. Lu, 27 : 700-, 2007

18 M. Golub, 8 : 21-, 2013

19 J. Amalric, 19 : 141-, 2009

20 C. -M. Tîlmaciu, 15 : 266-, 2015

21 H. Lee, 21 : 431-, 2009

22 I. -H. Bae, 23 : 1259-, 2012

23 G. Altankov, 5 : 732-, 1994

24 A. Curtis, 97 : 1500-, 1983

25 F. Schwarz, 35 : 64-, 2008

26 Y. Heo, 131 : 2014

27 S. Y. Seo, 128 : 4322-, 2013

28 D. Williams, "Titanium in Medicine" Springer 13-24, 2001

29 Bahman Nasiri-Tabrizi, "Production of poorly crystalline tricalcium phosphate nanopowders using different mechanochemical reactions" 한국공업화학회 20 (20): 1236-1242, 2014

30 박신혜, "Preparation of a Visible Light-Reactive Low Molecular-O-Carboxymethyl Chitosan (LM-O-CMCS) Derivative and Applicability as an Anti-Adhesion Agent" 한국고분자학회 19 (19): 921-927, 2011

31 이형재, "Preparation of Photoreactive Azidophenyl Hyaluronic Acid Derivative: Protein Immobilization for Medical Applications" 한국고분자학회 21 (21): 216-220, 2013

32 S. Anil, "Dental Implant Surface Enhancement and Osseointegration" InTech Open Access Publisher 2011