Prevalence of Centrocestus formosanus Metacercariae in Ornamental Fish from Chiang Mai, Thailand, with Molecular Approach Using ITS2

다국어 초록 (Multilingual Abstract)

The prevalence of Centrocestus formosanus metacercariae was investigated in ornamental fish purchased from a pet shop in Chiang Mai, Thailand, including Carassius auratus (goldfish), Cyprinus carpio (Koi), Poecilia latipinna (Sailfin Molly), Danio rerio (Zebrafish), and Puntigrus tetrazona (Tiger barb). The parasite species was identified by the morphology of worms as well as by a molecular approach using ITS2. The results showed that 50 (33.3%) of 150 fish examined were infected with the metacercariae. The highest prevalence was found in C. auratus (83.3%), and the highest intensity was noted in C. carpio (70.8 metacercariae/fish). The most important morphological character was the presence of 32-34 circumoral spines on the oral sucker. The phylogenetic studies using the rRNA ITS2 region revealed that all the specimens of C. formosanus in this study were grouped together with C. formosanus in GenBank database. This is the first report on ornamental fish, C. carpio, P. latipinna, D. rerio, and P. tetrazona, taking the role of second intermediate hosts of C. formosa-nus in Thailand. Prevention and control of metacercarial infection in ornamental fish is urgently needed.

참고문헌 (Reference)

1 Ortega C, "Trematode Centrocestus formosanus infection and distribution in ornamental fishes in Mexico" 21 : 18-22, 2009

2 Saenphet S, "The occurrence of heterophyid metacercariae in cyprinoid fish in Chiang Mai province" 39 : 56-61, 2008

3 Scholz T, "The introduction and dispersal of Centrocestus formosanus (Nishigori, 1924) (Digenea: Heterophyidae) in Mexico: a review" 143 : 185-200, 2000

4 Sukontason K, "Prevalence of trematode metacercariae in cyprinoid fish of Ban Pao District, Chiang Mai province, northern Thailand" 30 : 365-370, 1999

5 Krailas D, "Prevalence of fish-borne trematodes of the family Heterophyidae at Pasak Cholasid Reservoir, Thailand" 156 : 79-86, 2016

6 Evans BB, "Parasites of ornamental fish imported into Australia" 21 : 51-55, 2001

7 Waikagul J, "Opisthorchis viverrini metacercaria in Thai freshwater fish" 29 : 324-326, 1998

8 Radomyos B, "Opisthorchiasis and intestinal fluke infections in northern Thailand" 29 : 123-127, 1998

9 Wongsawad C, "Occurrence and molecular identification of liver and minute intestinal flukes metacercariae in freshwater fish from Fang-Mae Ai Agricultural Basin, Chiang Mai province, Thailand" 7 : 97-104, 2013

10 Saenphet S, "Mucosal mast cell responses in rats(Rattus norvegicus)experimentally infected with Centrocestus caninus" 37 : 446-451, 2006

11 Chalobol Wongsawad, "Molecular Phylogenetics of Centrocestus formosanus (Digenea: Heterophyidae) Originated from Freshwater Fish from Chiang Mai Province, Thailand" 대한기생충학ㆍ열대의학회 55 (55): 31-37, 2017

12 Gjurčević E, "Metacercariae of Centrocestus formosanus in goldfish (Carassius auratus L.) imported into Croatia" 4 : 214-216, 2007

13 Srisawangwong T, "Metacercariae isolated from cyprinoid fish in Khon Kaen district by digestion technique" 28 : 224-226, 1997

14 Pinto HA, "Melanoides tuberculata(Mollusca : Thiaridae)as an intermediate host of Centrocestus formosanus(Trematoda : Heterophyidae)in Brazil" 52 : 207-210, 2010

15 Tamaru K, "Mega6: molecular evolutionary genetics analysis version 6.0" 30 : 2725-2729, 2013

16 Keeseon S. Eom, "Infection Status of Zoonotic Trematode Metacercariae in Fishes from Vientiane Municipality and Champasak Province in Lao PDR" 대한기생충학ㆍ열대의학회 53 (53): 447-453, 2015

17 Mehrdana F, "Import of exotic and zoonotic trematodes (Heterophyidae: Centrocestus sp.) in Xiphophorus malculatus: implications for ornamental fish import control in Europe" 59 : 276-283, 2014

18 De NV, "Human infections of fish-borne trematodes in Vietnam: prevalence and molecular specific identification at an endemic commune in Nam Dinh Province" 129 : 355-361, 2011

19 Waikagul J, "Human infection of Centrocestus caninus in Thailand" 28 : 831-835, 1997

20 Wongsawad C, "Helminths of vertebrates in Mae Sa Stream, Chiang Mai, Thailand" 35 : 140-146, 2004

21 Mati LVT, "Experimental infection of Swiss and AKR/J mice with Centrocestus formosanus(Trematoda : Heterophyidae)" 55 : 13-36, 2013

22 Kimura D, "Epidemiological study on Centrocestus armatus metacercariae in the Chikusa River, Hyogo Prefecture, Japan" 33 : 7-11, 2005

23 Kimura D, "Development of Centrocestus armatus in different final hosts" 146 : 367-371, 2007

24 Chai JY, "Centrocestus formosanus(Heterophyidae) : human infections and the infection source in Lao PDR" 99 : 531-536, 2013

25 Mood SM, "Centrocestus formosanus metacercarial infection of four ornamental fish species imported into Iran" 30 : 146-149, 2010

26 한은택, "Centrocestus formosanus (Digenea: Heterophyidae) Encysted in the Freshwater Fish, Puntius brevis, from Lao PDR" 대한기생충학ㆍ열대의학회 46 (46): 49-53, 2008

27 Paller VGV, "Attachment and penetration of Centrocestus armatus(Digenea : Heterophyidae)cercariae to gills of secondary intermediate fish hosts" 93 : 578-583, 2008

28 Caron Y, "An optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails" 178 : 93-99, 2011

29 Boonchot K, "A survey of helminthes in cyprinoid fish from the Mae Ngad Somboonchon reservoir, Chiang Mai Province, Thailand" 36 : 103-107, 2005

30 Hong SJ, "A human case of Centrocestus armatus infection in Korea" 26 : 55-60, 1988