腦神經小膠細胞에서 생강 헥산 분획물의 염증매개물질 生成 抑制效果 = Hexane fraction of Zingiberis Rhizoma Crudus extract inhibits the production of nitric oxide and pro-inflammatory cytokines in LPS-stimulated BV2 microglial cells

다국어 초록 (Multilingual Abstract)

Excessive production of inflammatory mediators such as nitric oxide(NO), prostaglandin E2(PGE2), and proinflammatory cytokines, including tumor necrosis factor-alpha(TNF-α) and interleukin-1beta(IL-1β) from activated microglia contributes to uncontrolled inflammation in neurodegenerative diseases. It seems possible that treatment with anti-inflammatory agents, including plants used in Oriental medicine, might delay the progression of neurodegeneration through the inhibition of microglial activation. The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiberis Rhizoma Crudus(Ginger Hexan Extract; GHE) on the production of inflammatory mediators such as NO, PGE2, and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV2 cells, a mouse microglial cell line. GHE significantly inhibited the excessive production of NO, PGE2, TNF-α, and IL-1β in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2), and proinflammatory cytokines. The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases.

목차 (Table of Contents)

• Ⅰ. 서론 = 1
• Ⅱ. 실험 = 2
• 1. 재료 = 2
• 1) 배양세포 = 2
• 2) 약재 = 2
• 3) 시약 및 기기 = 2
• 2. 방법 = 2
• 1) 생강 헥산 분획물의 제조 = 2
• 2) 세포배양 = 3
• 3) 세포독성검정 = 3
• 4) NO 측정 = 3
• 5) PGE2 측정 = 4
• 6) Enzyme-linked immunosorbent assay(ELISA) = 4
• 7) Reverse Transcriptase-Polymerase Chain Reaction(RT-PCR) = 5
• 8) Western blot 분석 = 6
• 9) 통계 = 7
• Ⅲ. 결과 = 8
• 1. 세포독성검정 = 8
• 2. NO 생성억제효과 = 10
• 3. iNOS 유전자 및 단백질 발현 억제효과 = 12
• 4. PGE2 생성억제효과 = 14
• 5. COX-2 유전자 및 단백질 발현 억제효과 = 16
• 6. 전염증성 사이토카인 생성억제효과 = 18
• 7. 전염증성 사이토카인의 유전자 발현 억제효과 = 20
• Ⅳ. 고찰 = 22
• Ⅴ. 결론 = 26
• 참고문헌 = 27
• Abstract = 31