Effect of Oroxylum indicum on intestinal motility in rodents

다국어 초록 (Multilingual Abstract)

Oroxylum indicum is traditional herbal medicine in India, China and Japan used for its anti-diarrhoeal/anti-dysenteric activity, also found to be active against experimentally induced (DNBS induced) inflammatory bowel disease in rats with potential reduction in diarrhoea. It promotes us to evaluate effects of Oroxylum indicum on intestinal motility, both in vitro and in vivo, in rodents. Flavonoids rich fraction of O. indicum was obtained and the effect of extract on contraction of acetylcholine, barium chloride and electrical field stimulation was studied on isolated rabbit ileum. Anti-diarrhoeal activities were investigated using castor oil and magnesium sulphate-induced diarrhoeal models in mice. Effect on intestinal motility was studied using gastrointestinal motility and antienteropooling assay methods. Antimicrobial activity of extract was evaluated using disc diffusion assay method. Extract inhibited the contractions induced by acetylcholine, barium chloride and electrical field stimulation. Verapamil potentiates inhibitory effect of extract. Extract showed significant and dose-dependent antidiarrhoeal effect devoid of altering gastrointestinal motility in normal animals. It also inhibited the microbial growth in disc diffusion assay method. Extract normalized intestinal motility altered by inflammatory stimulus and possesses antidiarrhoeal activity. Alteration of intestinal motility may involve modification in L-type $Ca^{2+}$ channels.