Characterization of a Novel Cucumber mosaic virus Isolated from Petunia hybrida

다국어 초록 (Multilingual Abstract)

Petunia hybrida is commonly used in landscapes and interiors for its attractive flower. Virus-like foliar symptoms,including a mosaic with dark green islands surrounding the veins and chlorosis on the leaf margins,were observed on a petunia plant from Icheon, Gyeonggido,Korea. Cucumber mosaic virus (CMV) was identified in the symptomatic petunia by serological testing for the presence of CMV coat protein (CP) with a direct antibody-sandwich-enzyme-linked immunosorbent assay.
An agent was mechanically transmitted to indicator plant species including Chenopodium quinoa. Examination of the inoculated plant leaves by RT-PCR analysis and electron microscopy revealed the presence of specifically amplified CP products and spherical virions of approximately 28 nm in diameter, respectively, providing confirmation of a CMV infection. Analysis of CP sequences showed that CMV petunia isolate (CMVYJC)shared 82.5−100% amino acid sequence identity with CPs of representative CMV strains. Phylogenetic analysis of CPs supports that CMV-YJC is a member of CMV subgroup IA (CMV-IA) and has biological properties of CMV-IA on host species. To our knowledge,this is the first report of CMV from P. hybrida in Korea.

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