γ-Dodecelactone Production from Safflower Oil via 10-Hydroxy-12(<i>Z</i>)-octadecenoic Acid Intermediate by Whole Cells of <i>Candida boidinii</i> and <i>Stenotrophomonas nitritireducens</i>

다국어 초록 (Multilingual Abstract)

<P><I>Candida boidinii</I> was selected as a γ-dodecelactone producer because of the highest production of γ-dodecelactone from 10-hydroxy-12(<I>Z</I>)-octadecenoic acid among the 11 yeast strains tested. Under the reaction conditions of pH 5.5 and 25 °C with 5 g/L 10-hydroxy-12(<I>Z</I>)-octadecenoic acid and 30 g/L cells, whole <I>C. boidinii</I> cells produced 2.1 g/L γ-dodecelactone from 5 g/L 10-hydroxy-12(<I>Z</I>)-octadecenoic acid after 6 h, with a conversion yield of 64% (mol/mol) and a volumetric productivity of 350 mg/L/h. The production of γ-dodecelactone from safflower oil was performed by lipase hydrolysis reaction and two-step whole-cell biotransformation using <I>Stenotrophomonas nitritireducens</I> and <I>C. boidinii</I>. γ-Dodecelactone at 1.88 g/L was produced from 7.5 g/L safflower oil via 5 g/L 10-hydroxy-12(<I>Z</I>)-octadecenoic acid intermediate by these reactions after 8 h of reaction time, with a volumetric productivity of 235 mg/L/h and a conversion yield of 25% (w/w). To the best of the authors’ knowledge, this is the highest volumetric productivity and conversion yield reported to date for the production of γ-lactone from natural oils.</P><P><B>Graphic Abstract</B>
<IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2014/jafcau.2014.62.issue-28/jf501081z/production/images/medium/jf-2014-01081z_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf501081z'>ACS Electronic Supporting Info</A></P>