An Animal Study on the Nano Sized Hydroxy Apatite Contained Dentifrice for Dentinal Tubule Occlusion on the Rabbit Teeth

다국어 초록 (Multilingual Abstract)

The aim of this study was to evaluate dentinal tubule occlusion efficacy and safety of dentifrice containing nano sized Hydroxy Apatite for rabbit.
Methods: Subjects of this study were 15 of 14-weeks old male New Zealand white rabbits. For the study, rabbits were divided two groups of 7 and 14 day treatment. Upper and lower 4 incisors, totally 60 teeth were used for this experiment.
Experiment dentifrice 1 and 2 are prepared by containing 2% and 8% nano sized Hydeoxy Apatite respectively. For positive control, on-sale anti-hypersensitivity dentifrice which containing 19% of micro sized Hydroxy Apatite was used. Negative control was distilled water. For the safety evaluation, microscopic observation was performed for sacrificed rabbit’s gingival tissue.
Results: 1. Experimental 1, 2 and positive control dentifrice showed dentinal tubule occlusion efficacy (p＜0.001) and 14days treated experiment 1 and 2 groups showed higher occlusion effect than 7 days treatment (p＜0.01). 2. After 7 days treatment,experiment 1 and 2 dentifrice showed more effective dentinal tubule occlusion property than positive control dentifrice (p＜0.001). There is no significant difference between groups of experiment 1 and 2. 3. After 14-days treatment, dentinal tubule occlusion efficacy was higher in orderly experiment 2, experiment 1 and positive control dentifrice (p＜0.001). 4.
Any inflammation could not be found for all experimental gingival tissue samples.
Conclusion: Dentifrice containing nano-HAP showed higher dentinal tubule occlusion efficacy for rabbit than micro-HAP contained dentifrice. Dentinal tubule occlusion effect increased by nano-HAP content and treatment period. Dentifrice containing nano-HAP was safe for gingival tissue of rabbits.

다국어 초록 (Multilingual Abstract)

Objective: The aim of this study was to evaluate dentinal tubule occlusion efficacy and safety of dentifrice containing nano sized Hydroxy Apatite for rabbit.
Methods: Subjects of this study were 15 of 14-weeks old male New Zealand white rabbits. For the study, rabbits were divided two groups of 7 and 14 day treatment. Upper and lower 4 incisors, totally 60 teeth were used for this experiment.
Experiment dentifrice 1 and 2 are prepared by containing 2% and 8% nano sized Hydeoxy Apatite respectively. For positive control, on-sale anti-hypersensitivity dentifrice which containing 19% of micro sized Hydroxy Apatite was used. Negative control was distilled water. For the safety evaluation, microscopic observation was performed for sacrificed rabbit’s gingival tissue.
Results: 1. Experimental 1, 2 and positive control dentifrice showed dentinal tubule occlusion efficacy (p＜0.001) and 14days treated experiment 1 and 2 groups showed higher occlusion effect than 7 days treatment (p＜0.01). 2. After 7 days treatment,experiment 1 and 2 dentifrice showed more effective dentinal tubule occlusion property than positive control dentifrice (p＜0.001). There is no significant difference between groups of experiment 1 and 2. 3. After 14-days treatment, dentinal tubule occlusion efficacy was higher in orderly experiment 2, experiment 1 and positive control dentifrice (p＜0.001). 4.
Any inflammation could not be found for all experimental gingival tissue samples.
Conclusion: Dentifrice containing nano-HAP showed higher dentinal tubule occlusion efficacy for rabbit than micro-HAP contained dentifrice. Dentinal tubule occlusion effect increased by nano-HAP content and treatment period. Dentifrice containing nano-HAP was safe for gingival tissue of rabbits.

참고문헌 (Reference)

1 이수영, "나노 크기 탄산인회석을 함유한 치약의 상아세관 폐쇄효과 및 지속력 평가" 대한예방치과·구강보건학회 35 (35): 388-395, 2011

2 Rimondini L, "Ultrastructure of hypersensitive and non-sensitive dentine: a study on replica models" 22 (22): 899-902, 1995

3 김영회, "The Erosion of the Tooth Enamel and the Cementum by Carbonate Beverage" 대한예방치과학회 7 (7): 1-13, 2011

4 Jae-Hyun Ahn, "The Abrasive Effect of Toothpastes Containing Remineralization Components" 대한예방치과학회 8 (8): 215-223, 2012

5 Brannstrom M, "Sensitivity of dentin" 21 (21): 517-526, 1966

6 Marcacci M, "Reconstruction of extensive long-bone defects in sheep using porous hydroxyapatite sponges" 64 (64): 83-90, 1999

7 Chabanski MB, "Prevalence of cervical dentine sensitivity in a population of patients referred to a specialist Periodontology Department" 23 : 989-992, 1996

8 Hiatt WH, "Obturation of dentinal tubules in treatment of root hypersensitivity" 43 (43): 373-380, 1972

9 Son HH, "Flow of dentinal fluid through cat dentinal tubules in vivo" 20 (20): 342-350, 1995

10 Addy M, "Etiology and clinical implications of dentine hypersensitivity" 34 : 503-514, 1990

11 Kawasaki A, "Effects of plaque control on the patency and occlusion of dentine tubules in situ" 28 (28): 439-449, 2001

12 Ma DS, "Effect of the dentifrice containing hydroxyapatite, sodium pyrophosphate and carbamide peroxide on the reduction of gingivitis the change of microhardness and brightness on tooth enamel" 22 (22): 1-11, 1998

13 Absi EG, "Dentine hypersensitivity: a study of the patency of dentin tubules in sensitive and non-sensitive cervical dentine" 14 (14): 280-284, 1987

14 Addy M, "Dentine hypersensitivity: Its prevalence, aetiology and clinical management" 22 : 407-412, 1995

15 Absi EG, "Dentine Hypersensitivity-The development and evaluation of a replica technique to study sensitive and non-sensitivity cervical dentine" 16 (16): 190-195, 1989

16 Dowell P, "Dentin hypersensitivity-A review: Aetiology, symptoms and theories of pain production" 10 : 341-350, 1983

17 Baek DI, "Clinical preventive dentistry" Gomoonsa Press 255-264, 2011

18 Oh WM, "Changes in intradental nerve activity and occluding aspects of dentinal tubules by chemical desensitizing agents applied to the cat dentin" 20 (20): 508-526, 1995

19 Kim YS, "An animal study on apatite wollastonite glass ceramic for bio-material as fissure sealants" 14 (14): 297-307, 1990

20 Rees JS, "A cross sectional study of dentin hypersensitivity" 29 : 997-1003, 2002