항암성 생약제로 부터 항암성분의 추출 및 항암활성 측정 = Determination of Antitumor Effects of Extracts from Korean Herbal Drugs on Cancer cells

국문 초록 (Abstract)

암치료에 많이 사용되는 생약제 16종을 대상으로 항암활성을 규명하고자 in vitro에서 세포 독성능을 측정하여 다음과 같은 결과를 얻었다.
HEP 2 cell에 대해 세포 독성능을 높게보인 생약제는 하고초＞고삼＞화누＞선복화 순이었고, HeLa cell에 대해 세포독성능을 높게 보인 생약제는 선복화＞아출＞포공영＞화누＞구맥＞하고초＞고삼＞오공＞금은화＞천문동 순 이었다. RAW 71 cell에 대해 세포독성능을 높게 보인 생약제는 구맥＞화누＞고삼＞호모초＞천문동 순 이었고, VERO E6 cell에 대해 세포 독성능을 높게 보인 생약제는 선복화＞하고초 순 이었다.
또한 Herbal drug 처리에 의한 HEP 2와 HeLa 세포의 세포 독성능과 Staphylococcus aureus의 항균력간의 관계를 알아본 결과 HEP 2 cell은 약 43%, HeLa cell은 약 71%가 일치하였다.

다국어 초록 (Multilingual Abstract)

We tested the cytotoxic ability on the tumor cell line by 16 kinds of herbal drugs of which contains the alkaloids and flavoids which shows high antitumoral activity. This has been done on the condition of in vitro. the conclusion are as follows.
1. The herbal drugs which have shown the strong cytotoxic activity to HEP 2 cell are as follows. Prunella vulgaris has the strongest activity, followed by Sophorra angustifolia＞Trichoosanthes kirilowii＞Inula brontannaca.
2. The herbal drugs which have shown the strong cytotoxic activity to HeLa cell are as follows. Inula britannica has the strongest activity, followed by Curcuma zedoooaria＞Tarazacum platycarpum＞Trichosanthes kirilowii＞Dianthus sinesis＞Prunella vulgaris＞Sophra angustifolia＞Scolopendra subspinpes＞Lonicera Japonica＞Asparagus cochinensis.
3. the herbal drugs which have shown the strong cytotoxic activity to RAs 71 cell are as follows. Dianthus sinesis has the strongest activity, followed by Trichosanthes kirilowii＞Sophra angustifolia＞Corispermum stauntoni＞Asparagus cochinensis
4. The herbal drugs which have shown the strong cytotoxic activity to VERO E6 cell are as follows. Inula britannica has the strongest activity, followd by Prunella vulgaris.
5. After the treatment with herbal drugs, the relationshipbetween the cytotoxic activity to HEP 2 and HeLa cell, the antimicrobial activity of Staphylococcus aureus was found.
these two activites show the similarity of about 43% in HEP 2 cell and 71% in HeLa cell.