The effect of autogenous tooth bone graft material without organic matter and type I collagen treatment on bone regeneration

다국어 초록 (Multilingual Abstract)

Objectives: The aim of this study is to examine the effect of particulate autogenous tooth graft removed withorganic matter and type I collagen addition on bone regeneration and to validate the possibility of useful allograftmaterial for jaw defects.
Material and methods: Autogenous tooth bone maker (Korean Dental Solution® KOREA) made particulateautogenous tooth not including organic matter. We used to the developed tooth grafts for experiment. Celladhesion test with hemacytometer and energy dispersive X-ray spectroscopy (Supra40 VP®, Carl Zeiss, Germany)analysis about the particulate autogenous tooth and type I collagen were performed. Rabbits were divided intothree groups: bone graft with organic matter (OM) removing particulate autogenous tooth group, bone graft withOM removing particulate autogenous tooth and type I collagen group, and a control group. Bone grafting wasperformed in rabbit’s calvaria. The rabbits were sacrificed at different interval at 1, 2, 4, and 6 weeks after bonegrafting for the histopathologic observation and observed the effect of bone regeneration by SEM, H-E & Massonstains, osteocalcin IHC staining.
Result: In vitro cytopathological study showed affinity for cells, cell attachment pattern, and cell proliferation in theorder of control group, OM-removed and collagen-treated group, OM-removed particulate autogenous toothgroup. The results of the degree of mineralization were opposite to those of the previous cell experimental results,and the OM-removed group, OM-removed group and collagen-treated group were relatively higher than thecontrol group. Histopathologic analysis showed that vascularization and neonatal bone formation were higher inparticulate autogenous tooth group with removing OM and with addition of collagen than control group andgroup of OM removed only. Immunohistochemical analysis showed that osteocalcin (OSC) expression was notobserved in the control group, but at 4 weeks groups, OSC expression was observed the OM removed and OMremoved-collagen-treated particulate autogenous tooth, and the degree of expression was somewhat stronger ingroup of the OM removed and collagen additionally treated particulate autogenous tooth.

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