고지혈증 흰쥐에서 비배당체 이소플라본 고함유 대두분말의 혈청 지질 대사 영향과 항산화효과

국문 초록 (Abstract)

비배당체 이소플라본 고함유 대두분말이 고지방식이를 섭취한 흰쥐의 지질대사와 항산화 효과에 미치는 영향을 조사하고자 식이성 고지혈 흰쥐를 정상군(NO), 고지방식이군(CO), 고지방식이에 비배당체 이소플라본 고함유 대두분말 10%, 20%(w/w) 첨가군(BFF10, BFF20)으로 나누어 7주간 사육한 결과는 다음과 같다. 비배당체 이소플라본 고함유 대두분말 섭취는 체중과 간 무게를 유의적으로 감소시켰으며, CO군에 비해 혈청의 총 콜레스테롤, LDL-콜레스테롤, 중성지질 농도가 유의적으로 감소하였다. 간조직 손상시 활성증가가 우려되는 alanine amino transferase와 aspartate amino transferase 수준도 유의적으로 감소하였다. Thiobarbituric acid reactive substance의 생성량은 비배당체 이소플라본 고함유 대두분말의 첨가량이 높을수록 현저하게 감소되었으며 superoxide dismutase 활성은 농도 의존적으로 증가시키는 것으로 나타났다.

다국어 초록 (Multilingual Abstract)

This study was performed to evaluate the effects of soybean powder with rich aglycone isoflavone through bio-transformation on lipid metabolism and antioxidative activites in diet induced hyperlipidemic rats. The rats were randomly divided into four groups: NO (basal diet normal group), CO (high fat diet control group), BFF10 (soybean powder with rich aglycone isoflavone 10% group), and BFF20 (soybean powder with rich aglycone isoflavone 20% group). After 7 weeks of BFF10 or BFF20 diets consumption, the concentrations in serum triglyceride, total cholesterol, LDL-cholesterol, and atherogenic index ratios were significantly decreased in the BFF10 and BFF20 diets groups compared with those in the CO group. The activities of alanine amino transferase and aspartate amino transferase were significantly decreased in the BFF10 and BFF20 than those in the CO group. Thiobarbituric acid reactive substance levels of serum were decreased in BFF10 and BFF20 groups compared to that of the CO group. The super oxide dismutase activites were increased in BFF10 and BFF20 groups compared to that of the CO group.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• 감사의 글
• 문헌

참고문헌 (Reference)

1 주동식, "해조 올리고당 음료의 고지혈증 유도 쥐에 있어서 혈청 및 간장의 지질 변화에 미치는 영향" 한국식품영양과학회지 32 (32): 1364-1369, 2003

2 이윤나, "우리나라 성인의 체중상태에 따른 식생활 특성 -2001 국민건강.영양조사 결과에 근거하여" 대한지역사회영양학회 11 (11): 317-326, 2006

3 이명렬, "새싹채소 혼합분말이 고지방 식이를 급여한 흰쥐의 지질대사에 미치는 영향" 한국식품영양과학회지 36 (36): 965-974, 2007

4 이혜성, "고이소플라본 콩나물의 고지혈증 개선효과" 한국식품영양과학회지 36 (36): 1248-1256, 2007

5 송영옥, "β-carotene 첨가식이가 고지혈증 쥐의 지질과산화물과 항산화효소 활성에 미치는 영향" 한국영양학회 37 (37): 771-779, 2004

6 Smith EB, "The relationship between plasma and tissue lipids in human atherosclerosis" 11 : 1-49, 1974

7 Kim MJ, "The of dietary fiber on the lipid level and bowel function in rat" 28 : 23-32, 1995

8 Jeon KS, "The hydrolysis of isoflavones by Bifidobacterium sp. Int-57 during soymilk fermentation" 19 : 42-47, 2002

9 Haglund O, "The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in mans supplemented with vitamin" 121 : 165-172, 1991

10 Lee KH, "Soy isoflavone: current usage and production" 20 : 28-36, 2003

11 Messina M, "Soy intake and cancer risk. A review of in vitro and in vivo data" 21 : 113-131, 1994

12 Kang BH, "Reference value of hematology and serum chemistry in Ktc:Sprague-Dawley rats" 11 : 141-145, 1995

13 Moon SW, "Quality characteristics of chocolate blended with Bifidobacterium-fermented isofalvone powder" 6 : 1162-1168, 2003

14 Mitchell GV, "Protein efficiency ratios and net protein ratios of selected protein foods" 39 : 53-58, 1989

15 Bibgham SA, "Phytoestrogen; where are we now?" 79 : 393-406, 1998

16 Cassidy A, "Physiological effects of phytoestrogens in relation cancer and other human health risks" 55 : 399-417, 1996

17 Martin JP, "Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation" 255 : 329-336, 1987

18 Anderson JW, "Meta-analysis of the effects of soy protein intake on serum lipids" 333 : 276-282, 1995

19 Wang H, "Isoflavone content in commercial soybean foods" 42 : 1666-1673, 1994

20 Wei HC, "Inhibition of tumor promotor-induced hydrogen peroxid formation in vitro and in vivo by genistein" 20 : 1-12, 1993

21 Choi YB, "Hydrolysis of β-glycosidic bonds of isoflavone conjugates in the lactic acid fermentation of soy milk" 31 : 189-195, 1999

22 Picard F, "Effects of the estrogen antagonist EM-652.HCl on energy balance and lipid metabolism in ovariectomized rats" 24 : 830-840, 2000

23 Kim JD, "Effects of Meju powder supplementation on lipid metabolism in rats fed hypercholesterolemic diet" 26 : 314-318, 1997

24 Cha JY, "Effect of water extract of leaves from Moms alba and Cudrania tricuspidata on the lipid concentration of serum and liver in rats" 43 : 303-308, 2000

25 Kirk EA, "Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice" 128 : 954-959, 1998

26 Kwoon TW, "Current research on the bioactive functions of soyfoods in Korea" 15 : 1-12, 1998

27 Choi YS, "Cholesterol-lowering effect of soybean product (curd or crud residue) in rat" 22 : 673-677, 1993

28 Myant NB, "Cholesterol metabolism, LDL, and the LDL receptor" Academic Press Inc., 407-, 1990

29 Ohkawa H, "Assay for lipid peroxide in animal tissue by thiobabutiric acid reaction" 98 : 351-358, 1979

30 Morel D, "Antioxidative treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity" 30 : 1827-1834, 1989

31 Ruiz-Larrea MB, "Antioxidant activity of phytoestrgenic isoflavones" 26 : 63-70, 1997