<i>Agrobacterium</i>-mediated genetic transformation of radish (<i>Raphanus sativus</i> L.)

다국어 초록 (Multilingual Abstract)

<P>In order to generate transgenic radish (<I>Raphanus sativus</I> L., cv. Jin Ju Dae Pyong), hypocotyl explants were cultured on Murashige and Skoog medium containing 4 mg l<SUP>−1</SUP> AgNO<SUB>3</SUB>, 5 mg l<SUP>−1</SUP> acetosyringone, 4 mg l<SUP>−1</SUP> 6-benzyladenine, and 3 mg l<SUP>−1</SUP> α-naphthaleneacetic acid in addition to either 10 mg l<SUP>−1</SUP> hygromycin or 100 mg l<SUP>−1</SUP> paromomycin after co-cultivation with disarmed <I>Agrobacterium tumefaciens</I> harboring a plant expression binary vector. Explants co-cultivated with <I>A. tumefaciens</I> GV3101 harboring pCAMBIA1301 and <I>A. tumefaciens</I> EHA101 harboring pPTN290 produced putative transgenic adventitious shoots at frequencies of 0.26% and 0.18%, respectively. Northern blot analysis revealed the <I>gus</I> gene transcript was detected in 8 regenerated plants which confirmed their genetic transformation. The transgenic plants were grown to maturity after vernalization in a greenhouse and appeared morphologically normal. Progeny analysis of independent transgenic plants demonstrated that the <I>gus</I> gene was transmitted in a Mendelian pattern in 3 lines, indicating a single copied gene was incorporated into the genome.</P>