The effects of GABAergic drugs on trigeminal spinal sensory nucleus were studies in vitro. Transverse slices of caudal part of trigeminal sensory nucleus were prepared and perfused with aerated modified Krebs-Ringer solution. Intracellular recordings ...
The effects of GABAergic drugs on trigeminal spinal sensory nucleus were studies in vitro. Transverse slices of caudal part of trigeminal sensory nucleus were prepared and perfused with aerated modified Krebs-Ringer solution. Intracellular recordings were carried out with glass microelectrode to record the changes of membrane potential and membrane input resistance induced by GABA, baclofen, and bicuculline and glutamate perfusion. GABA and baclofen, a GABA_B agonist, did not influence significantly on the membrane potential and membrane input resistance. Bicuculline, a GABA_A antagonist, depolarized medullary dorsal horn neurons but lacked effects on membrane input resistance. Depolarizing effect of glutamate on medullary dorsal horn neurons was increased by simultaneous application of bicuculline.