An in vitro Microfluidic Gradient Generator Platform for Antimicrobial Testing

다국어 초록 (Multilingual Abstract)

Methicillin Resistant Staphylococcus pseu-dintermedius (MRSP) biofilm-related infections arecurrently a leading concern for veterinary hospitals, asthese types of infections are highly resistant to assaultsby both the immune system and antimicrobial thera-pies, impeding their clearance. Research suggests thatfosfomycin, a low molecular weight bactericidal anti-biotic, has the potential to effectively penetrate andsubsequently disrupt/destroy biofilm layers. Our studyutilized a fabricated microfluidic gradient generatorplatform as an assay to perform a quantitative assess-ment of varying concentrations of a selected antimi-crobial agent against MRSP biofilm formed under phy-siologically relevant conditions. Our results verifiedthe feasibility of using a microfluidic device for rapidantimicrobial testing against biofilms, which was suc-cessful in demonstrating that fosfomycin is an effec-tive agent that can disrupt established MRSP biofilms.
Additionally, Atomic Force Microscopy (AFM) analy-sis revealed that the cell walls of MRSP cells withinthe biofilms were disrupted by fosfomycin treatment,which speaks to the mechanism of action and the anti-microbial efficacy of this agent. This study providescompelling evident that microfluidic device and nano-scale AFM imaging-based investigations of biofilmscan aid in the study of biofilm-related infectious dis-eases.

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