發育鷄肝組織의 Cholesterol Esterifying Enzyme에 關한 硏究 = Studies on the Cholesterol Esterifying Enzyme in Developing Chick Liver

다국어 초록 (Multilingual Abstract)

The cholesterol esterifying enzyme activity in developing chick liver from 14th day of incubation to hatching and adult hen has been studied.
Chick livers from 14th day embryo and post-hatching were homogenized with 0.154 M phosphate buffer(pH 6.5) and incubated with oleate, sodium taurocholate, egg albumin and merthiolate for appropriated duration, in a shaker at 37℃, and then lipids were extracted by adding chloroform-methanol mixture.
The resutls obtained are as follows:
1. The activity of this enzyme was demonstrated in the liver at 14th day of incubation.
2. The liver of 14th day of incubation contained 4.0㎎ free cholesterol /g wet tissue. Similar values for the concentration of free cholesterol in the liver were found through the later phase of development and post natal period, except the livers of 21st day of incubation and of 1st day after hatching in which the free cholesterol contents have been shown to be 10.7㎎ and 40.5㎎/g wet tissue. respectively.
3. Cholesterol esterifying capacity of liver homogenate from 141th day of incubation has been shown to be approximately 9%, and was followed by a gradual increase during further incubation period. Immediately after hatching, the cholesterol esterifying capacity reached a maximum, (approx. 16%), then showed a slight decrease and reached the adult level. At 60th day after hatching the esterifying capacity was approximately.
4. The significance of activity of liver cholesterol esterifying enzyme is discussed in connection with the cholesterol ester concentration in developing chick liver.

목차 (Table of Contents)

• 緖論
• 實驗方法 및 材料
• 實驗成績 및 考察
• 結論
• 參考文獻