Detection of MAGE and SSX Gene Expressions by RT-nested PCR Using Common Primers in Head and Neck Cancer

다국어 초록 (Multilingual Abstract)

Objectives. The melanoma antigen gene (MAGE) and synovial sarcoma on X chromosome (SSX) gene families are silent
in most normal adult tissues, but are expressed in a variety of malignant lesions. Therefore, detection of MAGE and
SSX transcription may be useful for the diagnosis of head and neck cancers. The aim of this study is to detect
MAGE and SSX gene transcripts of head and neck cancers using the MAGE 1-6 assay and the SSX 1-9 assay.
Methods. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected by the MAGE 1-6 assay and the SSX 1-9 assay
respectively, in cancer cell lines, cancer tissue, and induced sputum specimens from head and neck cancer patients.
Results. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 82.8% and 75.9% of head and neck cancer tissues
(N=29) respectively, and 96.6% of cancer tissues expressed at least one of MAGE 1-6 or SSX 1-9 genes. In the
induced sputum of head and neck cancer patients (N=18), the transcripts of MAGE 1-6 and SSX 1-9 genes were
detected in 72.2% and 77.8%, respectively, and 94.4% of the sputum specimens were positive for either the MAGE
1-6 or the SSX 1-9 assay.
Conclusion. These results suggest that the combination of MAGE 1-6 and SSX 1-9 assays may be useful in the diagnosis
of head and neck cancer.

다국어 초록 (Multilingual Abstract)

Objectives. The melanoma antigen gene (MAGE) and synovial sarcoma on X chromosome (SSX) gene families are silent
in most normal adult tissues, but are expressed in a variety of malignant lesions. Therefore, detection of MAGE and
SSX transcription may be useful for the diagnosis of head and neck cancers. The aim of this study is to detect
MAGE and SSX gene transcripts of head and neck cancers using the MAGE 1-6 assay and the SSX 1-9 assay.
Methods. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected by the MAGE 1-6 assay and the SSX 1-9 assay
respectively, in cancer cell lines, cancer tissue, and induced sputum specimens from head and neck cancer patients.
Results. The transcripts of MAGE 1-6 and SSX 1-9 genes were detected in 82.8% and 75.9% of head and neck cancer tissues
(N=29) respectively, and 96.6% of cancer tissues expressed at least one of MAGE 1-6 or SSX 1-9 genes. In the
induced sputum of head and neck cancer patients (N=18), the transcripts of MAGE 1-6 and SSX 1-9 genes were
detected in 72.2% and 77.8%, respectively, and 94.4% of the sputum specimens were positive for either the MAGE
1-6 or the SSX 1-9 assay.
Conclusion. These results suggest that the combination of MAGE 1-6 and SSX 1-9 assays may be useful in the diagnosis
of head and neck cancer.

참고문헌 (Reference)

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