Selective Interaction Between Chloroplast β-ATPase and TGB1L88 Retards Severe Symptoms Caused by Alternanthera mosaic virus Infection

다국어 초록 (Multilingual Abstract)

The multifunctional triple gene block protein 1 (TGB1)of the Potexvirus Alternanthera mosaic virus (AltMV)has been reported to have silencing suppressor, cellto-cell movement, and helicase functions. Yeasttwo hybrid screening using an Arabidopsis thalianacDNA library with TGB1 as bait, and co-purificationwith TGB1 inclusion bodies identified several hostproteins which interact with AltMV TGB1. Hostprotein interactions with TGB1 were confirmed bybiomolecular fluorescence complementation, whichshowed positive TGB1 interaction with mitochondrialATP synthase delta′ chain subunit (ATP synthasedelta′), light harvesting chlorophyll-protein complex Isubunit A4 (LHCA4), chlorophyll a/b binding protein1 (LHB1B2), chloroplast-localized IscA-like protein(ATCPISCA), and chloroplast β-ATPase. However,chloroplast β-ATPase interacts only with TGB1L88,and not with weak silencing suppressor TGB1P88. Thisselective interaction indicates that chloroplast β-ATPaseis not required for AltMV movement and replication;however, TRV silencing of chloroplast β-ATPase inNicotiana benthamiana induced severe tissue necrosiswhen plants were infected by AltMV TGB1L88 but notAltMV TGB1P88, suggesting that β-ATPase selectivelyresponded to TGB1L88 to induce defense responses.

참고문헌 (Reference)

1 Strotmann, H., "in Advances in Photosynthesis Research" Martinus Nijhoff 477-484, 1984

2 Dinesh-Kumar, S. P., "Virus-induced gene silencing" 236 : 287-294, 2003

3 Verchot-Lubicz, J., "Varied movement strategies employed by triple gene block-encoding viruses" 23 : 1231-1247, 2010

4 Lim, H. S., "Triple gene block protein interactions involved in movement of Barley stripe mosaic virus" 82 : 4991-5006, 2008

5 Wasternack, C., "The wound response in tomato-role of jasmonic acid" 163 : 297-306, 2006

6 Chiu, M. -H., "The silencing suppressor P25 of Potato virus X interacts with Argonaute1 and mediates its degradation through the proteasome pathway" 11 : 641-649, 2010

7 Zhao, J., "The rubisco small subunit is involved in tobamovirus movement and Tm-2²-mediated extreme resistance" 161 : 374-383, 2013

8 Shinozaki, K., "The complete nucleotide sequence of the tobacco chloroplast genome: its gene organization and expression" 5 : 2043-2049, 1986

9 Matsushita, Y., "The catalytic subunit of protein kinase CK2 phosphorylates in vitro the movement protein of Tomato mosaic virus" 84 : 497-505, 2003

10 Jin, Y., "The HC-pro protein of Potato virus Y interacts with NtMinD of tobacco" 20 : 1505-1511, 2007

11 Fridborg, I., "TIP, a novel host factor linking callose degradation with the cell-to-cell movement of Potato virus X. Mol" 16 : 132-140, 2003

12 Samuels, T. D., "Subcellular targeting and interactions among the Potato virus X TGB proteins" 367 : 375-389, 2007

13 Strotmann, H., "Structure, function, and regulation of chloroplast ATPase" 35 : 97-120, 1984

14 Abbink, T. E., "Silencing of a gene encoding a protein component of the oxygen-evolving complex of photosystem II enhances virus replication in plants" 295 : 307-319, 2002

15 Raffaele, S., "Remorin, a Solanaceae protein resident in membrane rafts and plasmodesmata, impairs Potato virus X movement" 21 : 1541-1555, 2009

16 Perkins, D. N., "Probability-based protein identification by searching sequence databases using mass spectrometry data" 20 : 3551-3567, 1999

17 Qiao, Y., "Plastocyanin transit peptide interacts with Potato virus X coat protein, while silencing of plastocyanin reduces coat protein accumulation in chloroplasts and symptom severity in host plants" 22 : 1523-1534, 2009

18 Módena, N. A., "Phosphorylation of the TGBp1 movement protein of Potato virus X by a Nicotiana tabacum CK2-like activity" 137 : 16-23, 2008

19 Lee, J. Y., "Phosphorylation of movement proteins by the plasmodesmal-associated protein kinase" 451 : 625-639, 2008

20 Mathioudakis, M. M., "Pepino mosaic virus triple gene block protein 1 (TGBp1) interacts with and increases tomato catalase 1 activity to enhance virus accumulation" 14 : 589-601, 2013

21 Lim, H. S., "Pathogenicity of Alternanthera mosaic virus is affected by determinants in RNA-dependent RNA polymerase and by reduced efficacy of silencing suppression in a movement-competent TGB1" 91 : 277-287, 2010

22 Lakshman, D. K., "Optimized protein extraction methods for proteomic analysis of Rhizoctonia solani" 100 : 867-875, 2008

23 Groth, G., "New results about structure, function and regulation of the chloroplast ATP synthase (CF0CF1)" 106 : 142-148, 1999

24 Lim, H. S., "Mutation of a chloroplast-targeting signal in Alternanthera mosaic virus TGB3 impairs cell-to-cell movement and eliminates long-distance virus movement" 91 : 2102-2115, 2010

25 Waadt, R., "Multicolor bimolecular fluorescence complementation reveals simultaneous formation of alternative CBL/CIPK complexes in planta" 56 : 505-516, 2008

26 Schmid, J., "Molecular organization of the shikimate pathway in higher plants" 39 : 737-749, 1995

27 Mauch, F., "Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase" 25 : 67-77, 2001

28 Baulcombe, D. C., "Jellyfish green fluorescent protein as a reporter for virus infections" 7 : 1045-1053, 1995

29 Feki, S., "Interaction between tobacco ribulose-l,5-biphosphate carboxylase/oxygenase large subunit (RubisCO-LSU) and the PVY Coat Protein (PVY-CP)" 112 : 221-234, 2005

30 Jang, C., "Insights into Alternanthera mosaic virus TGB3Functions: Interactions with Nicotiana benthamiana PsbO Correlate with Chloroplast Vesiculation and Veinal Necrosis Caused by TGB3 Over-Expression" 4 : 5-, 2013

31 Bhat, S., "Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement" 161 : 134-147, 2013

32 Jiménez, I., "Identification of a plum pox virus CI-interacting protein from chloroplast that has a negative effect in virus infection" 19 : 350-358, 2006

33 Cheng, Y. Q., "HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in planta" 89 : 2046-2054, 2008

34 James, P., "Genomic libraries and a host strain designed for highly efficient two-hybrid selection in yeast" 144 : 1425-1436, 1996

35 Schmelz, E. A., "Fragments of ATP synthase mediate plant perception of insect attack" 103 : 8894-8899, 2006

36 Lim, H. S., "Efficiency of VIGS and gene expression in a novel bipartite Potexvirus vector delivery system as a function of strength of TGB1 silencing suppression" 402 : 149-163, 2010

37 Silhavy, D., "Effects and side-effects of viral RNA silencing suppressors on short RNAs" 9 : 76-83, 2004

38 Zhang, X., "Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense" 20 : 3255-3268, 2006

39 Schmelz, E. A., "Cowpea chloroplastic ATP synthase is the source of multiple plant defense elicitors during insect herbivory" 144 : 793-805, 2007

40 Dardick, C., "Comparative expression profiling of Nicotiana benthamiana leaves systemically infected with three fruit tree viruses" 20 : 1004-1017, 2007

41 Laemmli, U. K, "Cleavage of structural proteins during the assembly of the head of bacteriophage T4" 227 : 680-685, 1970

42 Caplan, J. L., "Chloroplastic protein NRIP1mediates innate immune receptor recognition of a viral effector" 132 : 449-462, 2008

43 Zhang, C., "Characterization of a specific interaction between IPL, a tobacco protein localized in thylakoid membranes, and Tomato mosaic virus coat protein" 374 : 253-257, 2008

44 Hammond, J., "An improved purification procedure for preparing potyviruses and cytoplasmic inclusion proteins from the same tissue" 20 : 203-217, 1988

45 남지륜, "AltMV TGB1 Nucleolar Localization Requires Homologous Interaction and Correlates with Cell Wall Localization Associated with Cell-to-Cell Movement" 한국식물병리학회 29 (29): 454-459, 2013

46 Shi, Y., "A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host" 8 : 785-790, 2007

47 Leshchiner, A. D., "A minimal region in the NTPase/helicase domain of the TGBp1 plant virus movement protein is responsible for ATPase activity and cooperative RNA binding" 87 : 3087-3095, 2006