Dunaliella tertiolecta 의 포도당산화와 산화효소계 (1) - Whole cells 과 cell - free systems 에 의한 14C - glucose 의 산화 = Glucose Oxidation and it's Oxidative Enzyme Systems in Dunaliella tertiolecta. (1) - Oxidation of 14C - glucose in Whole Cells and Cell - free Systems.

다국어 초록 (Multilingual Abstract)

Dunaliella tertiolecta did not show any increase in respiration rate when supplied with glucose, glycerol, sucrose, L-alanine, acetate, pyruvate and succinate. This was in contrast to Chlorella pyrenoidosa, which, under identical conditions, showed significant increase when supplied with glucose or acetate but not with the other compounds. Production of ^(14)CO_2 from added ^(14)C-glucose in D. tertiolecta was lower than the other ^(14)C-labelled substrates: L-alnine, glycerol, succinate, but higher than ^(14)C-sucrose addition. And it was also lower than C. pyrenoidosa experiments which were added ^(14)C-glucose as a substrate. Light reduced amounts of labelled carbon dioxide from ^(14)C-glucose or ^(14)C-acetate and increased incorporation of ^(14)C from the substrates to cell materials in either D. tertiolecta or C. pyrenoidosa. The contribution of ^(14)C from ^(14)C-glucose to ^(14)CO_2 in cell-free system of D. tertiolecta were much higher than in whole cell suspension. It was contrast to C. pyrenoidosa which were showed reduction of ^(14)CO_2 production in cell-free systems than whole cell suspensions. when cell-free systems of D. tertiolecta and C. pyrenoidosa were supplied with ATP, NAD, NADP or/and hexokinase, it was remarkably increased production of ^(14)CO_2 from the substrates than the control. It was concluded that the low ability of D. tertiolecta to metabolize glucose were caused by the impermeability of the cell membrane to glucose and were not due to deficiencies of enzyme systems concerning glucose metabolism. In the cell-free systems, it seemed to be more active pentose phosphate pathway than glycolytic pathway in D. tertiolecta.