A mice model of chronic non-healing wound and the dermal-equivalent for the assistance of re-epithelization process = A mice model of chronic non-healing wound and the dermal-equivalent for the assistance of re-epithelization process

다국어 초록 (Multilingual Abstract)

Background: Chronic wounds represent a significant burden to patients. Mice wound model is difficult to assess in that fast wound healing and different healing mechanism from human. Objectives: We propose a mouse burn skin model with “dermal equivalent(DE)” for the chronic non-healing wound and set appropriate schedule for wound evaluation in this model. Methods: To simulate chronic wound, we adopted a burn model. Six week-old nude mice are burnt on their back and the damaged skin was punched out at the center with remaining margins of burnt skin. Normal human dermal fibroblast (NHDF) were grown in vitro to build dermal equivalent (DE)”. Twenty four mice were divided into 6 groups according to the DE application and wound healing duration: wound only, wound with DE application, burnt wound only sacrificed at 16th day, burnt wound with DEsacrificed at 16th day, burnt wound only sacrificed at 12th day, and burnt wound with DE sacrificed at 12th day. We measured amount of skin contracture and final re-epithelization thickness to assess the effects of DE on the wound healing. Results: Wound contracture was significantly different in fresh wound groups, but not in both burnt wound groups, implying DE’s effect on wound healing was compromised.Interestingly, re-epithelization thickness was thicker in only DE applied group of burnt wound sacrificed at 12th day. Conclusion: The burn wound model in mice actually delayed wound healing. The wound evaluation should be done at least before 16th day.