한인진(Artemisia iwayomogi Kitamura)과 참당귀(Angelica gigas Nakai) 추출물이 넙치(Paralichthys olivaceus) 항병력에 미치는 영향 = Effects of Medicinal Herb Extracts of Artemisia iwayomogi Kitamura and Angelica gigas Nakai on Disease Resistance in Olive Flounder Paralichthys olivaceus

다국어 초록 (Multilingual Abstract)

Han In-jin (Artemisia iwayomogi Kitamura) and Cham Dang-gwi (Angelica gigas Nakai) exhibit antibacterial, antiparasitic, antifungal, and antiviral properties in vitro. In this study, mixture of the extracts of these two medicinal plants was absorbed on pellets. Thereafter, these pellets were fed to olive flounder Paralichthys olivaceus for 12 weeks at laboratory (1st experiment) and 24 weeks at field test (2nd experiment), and the immune activity and disease resistance properties of the extracts were examined. It was observed that lysozyme activities of plasma, spleen, and kidney improved after 12 weeks. Furthermore, when the olive flounders were artificially infected with bacterial pathogens, their cumulative mortality decreased in the group that was fed the extracts for 12 weeks compared to that in control group, and the relative percent survival also improved. This study concluded that mixture of Han In-jin and Cham Dang-gwi extracts provides disease resistance in vivo.

참고문헌 (Reference)

1 최혜승, "오배자 추출물의 어병 세균에 대한 항균 효능" 한국어병학회 18 (18): 239-245, 2005

2 김진도, "오매, Prunus mume 추출물 투여에 따른 뱀장어, Anguilla japonica의 면역 반응과 성장" 한국어병학회 22 (22): 367-374, 2009

3 강소영, "어류병원성 그람음성세균 Edwardsiella tarda와 Vibrio anguillarum에 대한 칠피의 항균활성물질" 한국어병학회 18 (18): 227-237, 2005

4 우승호, "어류 병원성 세균 공격 후 마늘, Allium sativum 착즙액의 침지가 넙치, Paralichthys olivaceus 의 면역반응에 미치는 영향" 한국어병학회 23 (23): 199-209, 2010

5 지보영, "양식 넙치 폐사피해 및 수산생물용 의약품 사용량 모니터링" 한국어병학회 27 (27): 77-83, 2014

6 손추영, "신약 개발을 위한 참당귀(Angelica gigas Nakai) 추출 Decursin과 Decursinol Angelate의 약리 작용" 대한약학회 53 (53): 300-313, 2009

7 전은지, "수산양식에서 친환경성 약제개발을 위한 항병성 생약소재의 검토" 한국수산해양교육학회 32 (32): 980-987, 2020

8 정승희, "손바닥선인장 줄기 가수분해 분획물의 어류 병원성 세균에 대한 항균활성" 한국어병학회 21 (21): 29-34, 2008

9 황지연, "생약제 첨가사료가 넙치의 백신효과에 미치는 영향" 한국수산해양교육학회 27 (27): 1491-1498, 2015

10 서정수, "생약제 고삼 뿌리 열수추출물의 넙치 투여시 질병 저항성에 미치는 영향" 한국수산해양교육학회 27 (27): 1656-1664, 2015

11 지보영, "생약재 황금 뿌리 열수추출물의 넙치 투여시 질병저항성에 미치는 영향" 한국어병학회 25 (25): 21-30, 2012

12 정승희, "생약재 첨가 사료를 투여한 넙치(Paralichthys olivaceus)의 비특이적 면역반응, 혈액성분 및 항병력 효과" 한국어병학회 15 (15): 25-35, 2002

13 이남실, "생약재 5종 열수추출물의 어류 병원체에 대한 in-vitro 약효와 선택된 한 종(황금)의 넙치에서의 항균효능과 독성시험" 한국어병학회 23 (23): 137-143, 2010

14 서정수, "복합 생약재 열수추출물 투여에 따른 넙치 세균성 질병에 대한 질병저항성" 한국수산해양교육학회 28 (28): 1715-1723, 2016

15 이준희, "마늘, Allium sativum이 넙치, Paralichthys olivaceus의 면역반응에 미치는 영향" 한국어병학회 23 (23): 69-83, 2010

16 김영재, "국내 수산용의약품의 관리 현황 모니터링" 한국어병학회 32 (32): 037-043, 2019

17 Ngo Van Hai, "The use of medicinal plants as immunostimulants in aquaculture: A review" Elsevier BV 446 : 88-96, 2015

18 A Newaj-Fyzul, "Probiotics, immunostimulants, plant products and oral vaccines, and their role as feed supplements in the control of bacterial fish diseases" Wiley 38 (38): 937-955, 2015

19 안종민, "Pharmaceutical Studies on “Dang-Gui” in Korean Journals" 한국생약학회 25 (25): 285-292, 2019

20 D. Christybapita, "Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus" Elsevier BV 23 (23): 840-852, 2007

21 Robertsen B, "Modulators of fish immune response (vol 1)" SOS Publication 83-99, 1994

22 M Krkosek, "Host density thresholds and disease control for fisheries and aquaculture" Inter-Research Science Center 1 (1): 21-32, 2010

23 Ramasamy Harikrishnan, "Herbal supplementation diets on hematology and innate immunity in goldfish against Aeromonas hydrophila" Elsevier BV 28 (28): 354-361, 2010

24 Jung SH, "Effect of oral administration by medicinal herb-extracts to non-specific immunity of olive flounder paralichthys olivaceus" 60 : 38-44, 2001

25 Ying-rui Wu, "Effect of Sophora flavescens on non-specific immune response of tilapia (GIFT Oreochromis niloticus) and disease resistance against Streptococcus agalactiae" Elsevier BV 34 (34): 220-227, 2013

26 Qing-Kui Wang, "Dietary polysaccharide from Angelica sinensis enhanced cellular defence responses and disease resistance of grouper Epinephelus malabaricus" Springer Science and Business Media LLC 19 (19): 945-956, 2011

27 Seung-Cheol JI, "Dietary medicinal herbs improve growth performance, fatty acid utilization, and stress recovery of Japanese flounder" Springer Science and Business Media LLC 73 (73): 70-76, 2007

28 Osamu Takaoka, "Dietary medicinal herbs and enzyme treated fish meal improve stress resistances and growth performance at early juvenile stage of red sea breamPagrus major" Wiley 47 (47): 390-397, 2016

29 László Ardó, "Chinese herbs (Astragalus membranaceus and Lonicera japonica) and boron enhance the non-specific immune response of Nile tilapia (Oreochromis niloticus) and resistance against Aeromonas hydrophila" Elsevier BV 275 (275): 26-33, 2008

30 Hoseinifar SH, "Boosting immune function and disease bio-control through environment-friendly and sustainable approaches in finfish aquaculture : Herbal therapy scenarios" 28 : 303-321, 2020

31 Mok JS, "Antibacterial effect of fish diet soaked in Salvia miltiorriza extract" 14 : 157-163, 2001

32 Mok JS, "Antibacterial activity of ethanol extract of root bark of Morus alba against selected fish pathogenic bacteria" 14 : 221-226, 2001

33 Zhu F, "A review on the application of herbal medicines in the disease control of aquatic animals" 526 : 735422-, 2020

34 R. M. Parry, "A Rapid and Sensitive Assay of Muramidase" SAGE Publications 119 (119): 384-386, 1965

35 MFDS (Ministry of Food and Drug Safety), "13-week repeated dose toxicity and genotoxicity study of angelicae radix 2005" MFDS 1-77, 2005