Effects of Propolis on Sperm Function in Frozen-thawed Boar Semen

다국어 초록 (Multilingual Abstract)

The aim of this study was to evaluate effect of Propolis on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. The boar semen was collected by gloved-hand method and cryo-preserved in 11% lactose with 20% egg yolk (LEY) and Triladyl containing propolis (0, 10, 30, and 50 ng/mL) with 0.05% ethanol. Cryo-preserved boar sperms was thawed at 37.5℃ for 45 sec in water-bath. Sperm viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed sperm, which was cryo-preserved using LEY and triladyl, was decreased in 0, 30, and 50 propolis treatment groups. However, there was no significant difference. Acrosome reaction and mitochondrial intact of sperm was not influenced by all of propolis treatment using both of freezing extender. Motility of frozen-thawed sperm with LEY containing 10 ng/mL propolis was significantly higher than in 0, 10, and 50 ng/mL propolis treatment groups (p<0.05), whereas there was no significant difference compared to control. Especially, 0 and 30 ng/mL propolis treatment groups were significantly decreased compared to control (p<0.05). Similar to result in frozen-thawed sperm with LEY, motility of frozen-thawed sperm with triladyl was significantly increased in 10 ng/mL propolis treatment groups compare to control and other propolis treatment groups (p<0.05). In conclusion, 10 ng/mL propolis improves mobility in frozen-thawed boar sperm with LEY and triladyl, whereas 30 and 50 ng/mL propolis reduced motility. But, the addition of propolis during cryopreservation did not influence to the membrane function of frozen-thawed spermatozoa. Therefore, it is necessary to continue the study on the effects of propolis on boar spermatozoa during cryopreservation.