A Systematic Proteome Study of Seed Storage Proteins from Two Soybean Genotypes

다국어 초록 (Multilingual Abstract)

Soybean seed is a good source of plant proteinin human consumables such as baby formula and proteinconcentrate. The seeds contain an abundance of storageproteins, namely β-conglycin and glycinin that account for ~70-80% of the total seed protein content. Proteome profilinghas been proved to be an efficient way that can help usto investigate the seed storage proteins. In the present study,the seeds were removed from the pods and the cotylendonarytissues were separated from the testa for proteome analysisin order to investigate the seed storage proteins. A systematicproteome profiling was conducted through one-dimensionalgel electrophoresis followed by MALDI-TOF-TOF massspectrometry in the seeds (cotyledonary tissue) of soybeangenotypes. Two dimensional gels stained with CBB, a totalof 10 proteins were identified and analyzed using MASCOTsearch engine according to the similarity of sequences withpreviously characterized proteins along with the UniProtdatabase. A total of ten proteins such as glycinin Gy4 precursor,glycinin G3 precursor, glycinin G1 precursor, glycinin chainA2B1a precursor, glycinin chain A2B1a precursor wereidentified in our investigation. However, the glycinin subunitmay be considered to play important roles in soybean breedingand biochemical characterization. In addition, the improvedtechnique will be useful to dissect the genetic control ofglycinin expression in soybean

참고문헌 (Reference)

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