흰쥐에서 SAL5의 알코올성 지방간 형성에 미치는 영향 = Effect of SAL5 on chronic ethanol-induced fatty liver model

다국어 초록 (Multilingual Abstract)

Objective : In this study, we investigated the effect of SAL5(mixing extracts of Schisandra chinensis Baillon, Artemisia capillaris Thunb., and Aloe vera Linne) on chronic ethanol-induced fatty liver model.
Methods : Sprague-Dawley male rats were fed Liber-DeCarli (normal), ethanol liquid diet (control), SAL5 (200 ㎎/㎏). We administrated the SAL5 on chronic ethanol-induced fatty liver model for 5 weeks. We measured alkaline phosphtase (ALP), alanine transminase (ALT), aspartate transminase (AST) and γ-glutamyl transpeptase (γ-GTP) in serum and triglyceride (TG), superoxide dismutase (SOD), catalase, glutathione (GSH) and malondialdehyde (MDA) level in liver.
Liver histopathology was examined by Hematoxylin-eosin and Oil red O staining of the fixed liver tissues. Real-time PCR was performed to measure the mRNA expression of inflammatory cytokines and MMP-2, MMP-9.
Results : SAL5 administration resulted in significantly decreased liver marker enzymes activities of alanine transminase (ALT), γ-glutamyl transpeptase (γ-GTP) in serum and triglyceride (TG) activities in liver. The control group decreased the activities of superoxide dismutase (SOD), catalase (CAT) with the reduced level of glutathione (GSH) in liver. On the other hand, SAL5 group increased the activities of SOD, CAT and the level of GSH. SAL5 delayed the development of an alcoholic fatty liver by reversing fat accumulation in the liver, as evidenced in histological observations. The gene expression of mRNA were significantly decreased at the IL-1β, TNF-α, NOS-II and MMP-2 by SAL5.
Conclusions : These results indicate that SAL5 might have protective effect chronic ethanol-induced fatty liver models.

참고문헌 (Reference)

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