꿀벌부채명나방의 저온조건에 의한 단백질의 함량 변화 및 전기영동적 패턴 = Electrophoretic Patterns and Protein Contents in the Great wax moth, Galleria mellonella by Low Temperature Condition

국문 초록 (Abstract)

함량의 변화와 아미노산의 함량을 조사하였다.
단백질 패턴은 모든 온도조건을 통해 조직과 혈림프에서 각각 30개와 34개가 분리되었고, 최고 분자량은 146,520 daltons, 최저 분자량은 27,390 daltons으로 나타났다.
분자량이 58,490 daltons 단백질은 0℃에서만 분리되고 높은 염색강도를 나타내어 특이하게 여겨진다.
분자량이 45,100∼31,590 daltons 사이에서 단백질의 패턴변화가 심하게 나타났다.
단백질의 함량이 혈림프와 조직에서는 저온에서 증가한 반면 아미노산은 감소하였다.
이상의 결과로 보아 저분자 단백질은 조직에서 새로이 축적되고 고분자 단백질은 혈림프에 나타난다고 여겨진다. 또한 전체적으로 온도가 낮을수록 단백질은 혈림프에 축적된다고 여겨진다.

다국어 초록 (Multilingual Abstract)

The change of protein patterns and protein, amino acid contents in tissue and hemolymph of the great wax moth, Galleria mellonella according to low temperature condition were determined.
Throughout all temperature condition, the patterns were separated into each 30 bands and 34 bands in tissue and hemolymph.
The highest molecular weights was 146,520 daltons and the lowest one weight was 27,390 daltons.
We were uniquely thought 58,490 daltons proteins were separated into only at 0℃ and were showed high colar density.
The changes of protein patterns were extremely appeared between 45,100 daltons and 31,590 daltons.
In hemolymph and tissue, protein contents was increased at low temperature but amino acid contents was decreased.
In conclussion, low molecular protein were newly accumulated in tissue and high molecular proteins were appeared in hemolymph.
In general, proteins were accumulated in hemolymph as a temperature decreased.