대장샘암종에서예후인자로서의Fascin, MMP-9 과발현과 Proliferating Index에 대한 면역조직 화학적 연구 = Immunohistochemical Studies of Fascin, MMP-9 Overexpression, and Proliferating Index as Prognostic Factors in Cases of a Colon Adenocarcinoma

다국어 초록 (Multilingual Abstract)

Purpose: The malignant conversion of epithelial cells involves alterations in the expression and the function of cell-matrix and
cell-cell adhesive systems that enable a switch to a migratory phenotype in tumor invasion and metastasis. Here, the author
studies the prevalence and the potential clinical significance of fascin and Matrix metalloproteinase-9 (MMP-9) expression
in relation to the progression of colon adenocarcinoma and of tumor cell proliferation as measured by using the topoisomerase
II-α(Topo II-α) index.
Methods: Relatively well-preserved paraffin-embedded tissues of 120 cases of colon adenocarcinomas were immunohistochemically
stained for fascin, MMP-9, and Topo II-αexpression. A reaction was determined as being positive when more
than 10% of the cells were positive for fascin, and/or MMP-9. The Topo II-αindex is defined as the positive number of tumor
cells divided by the total number of tumor cells counted times 100. At least 1,000 cells were counted for this analysis. A χ2 test,
by using Epi info 2000, for Fascin and/or MMP-9 and a two-sided test for the Topo II-αindex were employed with a significance
of P<0.05.
Results: Positive reactions for fascin and MMP-9 in colon adenocarcinomas were 44.2% and 56.7%, respectively. In clinically
annotated tumors, fascin immunoreactivity was more common in tumors located in the right colon (P=0.014) and was associated
with older age (>65 yr, P=0.028), tumor grading (P=0.009), and lymph node metastases (P=0.005). However, MMP-9 immunoreactivity
was not statistically associated with age, gender, tumor stage, or lymph node metastases. Fascin expression
was statistically associated with MMP-9 expression, especially for left colon adenocarcinomas (P=0.0032). Although the topo
II-αproliferating index was associated with lymph node metastasis (P<0.01), this result was not statistically associated with
Fascin or MMP-9 expression.
Conclusion: Fascin expression may be closely linked with tumor grading and lymph node metastasis of more aggressive
colon adenocarcinomas and partly associated with MMP-9 expression in tumor invasion. However, further studies of fascin
expression as an independent prognostic factor are required for the determination of significant relationships with other clinicopathologic
indices.

다국어 초록 (Multilingual Abstract)

Purpose: The malignant conversion of epithelial cells involves alterations in the expression and the function of cell-matrix and
cell-cell adhesive systems that enable a switch to a migratory phenotype in tumor invasion and metastasis. Here, the author
studies the prevalence and the potential clinical significance of fascin and Matrix metalloproteinase-9 (MMP-9) expression
in relation to the progression of colon adenocarcinoma and of tumor cell proliferation as measured by using the topoisomerase
II-α(Topo II-α) index.
Methods: Relatively well-preserved paraffin-embedded tissues of 120 cases of colon adenocarcinomas were immunohistochemically
stained for fascin, MMP-9, and Topo II-αexpression. A reaction was determined as being positive when more
than 10% of the cells were positive for fascin, and/or MMP-9. The Topo II-αindex is defined as the positive number of tumor
cells divided by the total number of tumor cells counted times 100. At least 1,000 cells were counted for this analysis. A χ2 test,
by using Epi info 2000, for Fascin and/or MMP-9 and a two-sided test for the Topo II-αindex were employed with a significance
of P<0.05.
Results: Positive reactions for fascin and MMP-9 in colon adenocarcinomas were 44.2% and 56.7%, respectively. In clinically
annotated tumors, fascin immunoreactivity was more common in tumors located in the right colon (P=0.014) and was associated
with older age (>65 yr, P=0.028), tumor grading (P=0.009), and lymph node metastases (P=0.005). However, MMP-9 immunoreactivity
was not statistically associated with age, gender, tumor stage, or lymph node metastases. Fascin expression
was statistically associated with MMP-9 expression, especially for left colon adenocarcinomas (P=0.0032). Although the topo
II-αproliferating index was associated with lymph node metastasis (P<0.01), this result was not statistically associated with
Fascin or MMP-9 expression.
Conclusion: Fascin expression may be closely linked with tumor grading and lymph node metastasis of more aggressive
colon adenocarcinomas and partly associated with MMP-9 expression in tumor invasion. However, further studies of fascin
expression as an independent prognostic factor are required for the determination of significant relationships with other clinicopathologic
indices.

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