In this work, we demonstrate that controlled dual release of bone morphogenic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) by catechol-functionalized adhesive polymer nanoparticles on microgrooved titanium (Ti) surface enhances in vitro ...
In this work, we demonstrate that controlled dual release of bone morphogenic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) by catechol-functionalized adhesive polymer nanoparticles on microgrooved titanium (Ti) surface enhances in vitro osteoblastic differentiation of human mesenchymal stem cells (MSCs). The nanoparticles were immobilized both on the groove bottom surface and the ridge top surface. A fluorescence microscope visualized that BMP-2 and IGF-1 of positive charges were efficiently loaded onto the nanoparticle-immobilized Ti surfaces. We confirmed the enhanced osteoblastic differentiation of MSCs by presenting the expression results of major osteoblast marker genes and proteins. The proposed combined surface of microgrooves and growth factor-releasing nanoparticles can be used as a strong osteogenic promoter on various biomaterial surfaces.