제주 자생 조록나무 분획물의 항산화 효과 = Antioxidant Activity of Solvent Fractions from Distylium racemosum in Jeju

국문 초록 (Abstract)

600여 종의 제주 천연 추출물을 이용하여 항산화 효과를 스크린한 결과, 조록나무 잎 추출물이 가장 높은 항산화 효과를 나타내었다. 조록나무는 조록나무과에 속하는 상록 나무로써 제주 천연자원이다. 본 연구에서는 극성이 다른 유기용매(헥산, 메틸렌 클로라이드, 에틸 아세테이트, 부탄올, 물)로 순차적으로 분획 한 조록나무물질 5가지를 이용하여 항산화 효과 및 간세포와 폐세포를 이용하여 세포 보호능을 확인하였다. 에틸 아세테이트 분획물질은 0.5mg/mL에서 약93%로 가장 우수한 DPPH 라디칼 소거능을 보이며 양성 대조군으로 사용한 quercetin 보다 높은 효능을 보였다. 총 페놀 함량을 확인 한 결과, 에틸 아세테이트 분획물질은 505 mg GAE/g로 가장 높은 총 페놀 함량을 보였으며, 모든 추출물에서 DPPH 라디칼 소거능과 연관성 있는 결과를 보였다. 또한 에틸 아세테이트 분획물질은 H2O2에 대한 산화적 스트레스로부터 다른 분획 물질보다 높은 세포보호효과를 나타내었다. 본 연구를 통해 조록나무의 에틸 아세테이트 분획물질의 높은 항산화 효과를 확인하였으며, 천연 항산화 소재의 가능성이 높을 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

Anti-oxidant activity of 600 medicinal plants from Jeju was analyzed. Extracts from the leaves of Distylium racemosum have the highest anti-oxidant activity. D. racemosum is an oriental medicinal plant belonging to the Hamamelidacea and grows in the wild in Jeju. This study was conducted to evaluate the antioxidant and cell viability of different fractions (n-hexane, methylene chloride, ethyl acetate, buthanol, DW) from D. racemosum. Anti-oxidant activity was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test and total phenolic content. Cell viability was determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) cell viability assay on HepG2 and A549 cells. Among various extracts, the ethyl acetate fraction showed the highest DPPH radical scavenging activity, reaching approximately 93% at 0.5 mg/mL, higher than that of quercetin used as a positive control. Ethyl acetate fractions showed the highest total phenolic content at 505 mg GAE/g. The phenolic content of each extract showed association with DPPH radical scavenging activity. The ethyl acetate extracts were resistant against hydrogen peroxide (H2O2) treatment in the MTT cell viability assay and showed a higher cell protective effect than other fraction extracts. These results suggest that the ethyl acetate fraction might be a source of anti-oxidants of D. racemosum.

참고문헌 (Reference)

1 임요섭, "배양된 C6 신경교종세포의 세포생존능과 항산화 효과에 순천만에서 자란 퉁퉁마디의 물리적활성의 연구" 대한임상검사과학회 43 (43): 98-104, 2011

2 Moon JY, "Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants" 4 : 41-53, 2010

3 원새봄, "Protective Effect of Pinus koraiensis Needle Water Extract Against Oxidative Stress in HepG2 Cells and Obese Mice" 한국식품영양과학회 16 (16): 569-576, 2013

4 Kim JA, "New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H" 21 : 2840-2844, 2011

5 Ryeo Kyeong Ko, "New Dibenzofurans from the Branches of Distylium racemosum Sieb. et Zucc" 대한화학회 30 (30): 1376-1378, 2009

6 김경미, "Intestinal anti-inflammatory activity of Sasa quelpaertensis leaf extract by suppressing lipopolysaccharide-stimulated inflammatory mediators in intestinal epithelial Caco-2 cells co-cultured with RAW 264.7 macrophage cells" 한국영양학회 9 (9): 3-10, 2015

7 Chipiti T, "In vitro antioxidant activities of leaf and root extracts of Albizia antunesiana harms" 70 : 1035-1043, 2013

8 Chen CJ, "Hepatoprotective activity of Cichorium endivia L. extract and its chemical constituents" 16 : 9049-9066, 2011

9 Lampe JW, "Health effects of vegetables and fruit: assessing mechanisms of action in human experimental studies" 70 (70): S475-S490, 1999

10 Singh N, "Free radical scavenging activity of an aqueous extract of potato peel" 85 : 611-616, 2004

11 Hong JH, "Effects of Artemisia capillaris ethyl acetate fraction on oxidative stress and antioxidant enzyme in high-fat diet induced obese mice" 179 : 88-93, 2009

12 Ko RK, "Compounds with tyrosinase inhibition, elastase inhibition and DPPH radical scavenging activities from the branches of Distylium racemosum Sieb. et Zucc" 25 : 1451-1456, 2011

13 Jullian C, "Complexation of quercetin with three kinds of cyclodextrins : an antioxidant study" 67 : 230-234, 2007

14 제갈승주, "C57BL/6 마우스에서 고지방 식이와 CCl4로 유발한 간섬유증에 미치는 커큐민의 보호효과" 대한임상검사과학회 47 (47): 251-258, 2015

15 Ferrer P, "Association between pterostilbene and quercetin inhibits metastatic activity of B16 melanoma" 7 : 37-47, 2005

16 Duthie G, "Antioxidant capacity of flavonoids in hepatic microsomes is not reflected by antioxidant effects in vivo" 2012 : 165127-, 2012

17 Mahakunakorn P, "Antioxidant and free radical-scavenging activity of Choto-san and its related constituents" 27 : 38-46, 2004

18 Bayati S, "Antioxidant and free radical scavenging potential of yakuchinone B derivatives in reduction of lipofuscin formation using H2O2-treated neuroblastoma cells" 15 : 134-142, 2011

19 Lee SM, "Antioxidant activity of two phloroglucinol derivatives from Dryopteris crassirhizoma" 26 : 1354-1356, 2003

20 Kumarappan CT, "Antioxidant activity of polyphenolic extracts of Ichnocarpus frutescens" 19 : 349-355, 2012

21 Debnath T, "Anti-oxidant and anti-inflammatory activities of Inonotus obliquus and germinated brown rice extracts" 18 : 9293-9304, 2013

22 Jo HJ, "Analysis of anti-oxidant activity of medicinal plants according to the extracted parts" 7 : 1089-1099, 2013

23 Piao MJ, "An ethanol extract derived from Bonnemaisonia hamifera scavenges ultraviolet B(UVB)radiation-induced reactive oxygen species and attenuates UVB-induced cell damage in human keratinocytes" 10 : 2826-2845, 2012