대금음자가미와 이(梨)추출물 배합제제가 알코올로 유발된 간손상에 미치는 영향 = Effects of combination pear extract with Daekumeumjagami medication on hepatic injury induced by alcohol in mice

다국어 초록 (Multilingual Abstract)

Objectives : The effect of pear extract with Daekumeumjagami and vitamin C medication(PDV) on alcohol metabolism and hepatic injury was assessed following hepatic injury induced by alcohol in mice. Methods : The model of alcoholic hepatic injury was established by orally administration with 3 g/kg 25% alcohol in mice. PDV was orally administrated once a day for 5 days. Mice were randomly divided into 5 groups : normal group, control group, and PDV groups (PDV-A, PDV-B and PDV-C). The activities of aspartate amino transferase (AST) and alanine amino transferase (ALT) and alcohol dehydrogenase (ADH) in serum, superoxide dismutase (SOD) and catalase in liver were determined after alcohol exposure. Results : Compared with control group, treatment with PDV-B and PDV-C significantly elevated activities of ADH. Moreover, the index of hepatic injury in serum was significantly decreased by treatment with PDV-B and PDV-C in ALT activity and PDV-C in AST activity. Additionally, enhanced catalase activities in liver was found in PDV-C treated mice after exposure to alcohol. Also, WBC in blood was significantly lower by treatment with PDV-B and PDV-C. Conclusions : This study suggests that PDV treatment could enhance alcohol metabolism, and prevent hepatic injury after alcoholic hepatic injury and that this effect is likely related to its modulation on the alcohol metabolizing and antioxidant enzymes.

참고문헌 (Reference)

1 정성제, "약용 식물 추출물의 항산화 활성 검색" Applied Biological Chemistry 47 (1) : 135 ~ 140 , 2004

2 김영식, "알코올 및 사염화탄소에 의해 유발된 급만성 간손상에 대한 지구자 열수추출물의 보호효과" 대한본초학회지 28 (4) : 25 ~ 32 , 2013

3 김정상, "생지황(Rehmanniae Radix)과 배의 Phenolic Compound가 Streptozotocin으로 유발된 고혈당 생쥐에 미치는 영향" 한국식품영양과학회지 33 (1) : 66 ~ 71 , 2004

4 김왕인, "배추출물과 구기자, 의이인, 택사, 황기 추출물 배합이 고지방식이로 유발된 비만 백서의 체중, 지질대사, 염증 및 면역기능에 미치는 영향 (Ⅱ)" 대한본초학회지 27 (5) : 1 ~ 7 , 2012

5 김왕인, "배추출물과 구기자, 의이인, 택사, 황기 배합이 고지방식이로 유발된 비만 흰쥐의 체중, 지질대사 및 면역기능에 미치는 영향 (Ⅰ)" 대한본초학회지 27 (3) : 7 ~ 13 , 2012

6 나창수, "배에서 추출된 Pectin과 Phenolic compound가 흰쥐의 국소뇌혈류 및 심혈관계에 미치는 영향" 대한본초학회지 18 (2) : 9 ~ 108 , 2003

7 나창수, "배 추출 펙틴이 2K1C 고혈압 흰쥐의 혈압, 혈장 Renin, ANP 및 Cardiac Hypertrophy에 미치는 영향" 한국식품영양과학회지 32 (5) : 700 ~ 705 , 2003

8 이참결, "대금음자 약침이 알코올 독성 흰쥐의 해마에서 c-Fos 발현에 미치는 영향" 대한침구의학회지 23 (3) : 37 ~ 45 , 2006

9 김동춘, "녹차추출물이 에탄올 투여에 의한 초기 간 손상에 미치는 영향" 한국식품영양과학회지 39 (3) : 343 ~ 349 , 2010

10 김현중, "對金飮子 약침이 알코올 독성 흰쥐의 해마에서 신경세포생성과 NOS 발현에 미치는 영향" 대한침구의학회지 23 (5) : 187 ~ 198 , 2006

11 Hwang TH, "The effects of Daigeumeumja against the mucosal injury of small intestine by ethanol and stress in mice" J Kor Oriental Intern Med 24 (4) : 735 ~ 746 , 2003

12 Weizhong, "Taipinghuiminhejijufang" Zhongguo Zhongyiyao Press : 48 ~ 49 , 1996

13 Lee JS, "Supplementation of Pueraria radix water extract on changes of antioxidant enzymes and lipid profile in ethanol-treated rats" Clin Chim Acta 347 (1-2) : 121 ~ 128 , 2004

14 Molander DW, "Serum glutamic oxalacetic transaminase as an index of hepatocellular integrity" J Lab Clin Med 46 (6) : 831 ~ 839 , 1955

15 Baudrimont I, "Prevention of lipid peroxidation induced by ochratoxin A in Vero cells in culture by several agents" Chem Biol Interact 104 (1) : 29 ~ 40 , 1997

16 Jeong DH, "Physiological Function of Food" Sunjin Publishers : 109 ~ 112 , 1998

17 Wu D, "Oxidative stress and alcoholic liver disease" Semin Liver Dis 29 (2) : 141 ~ 154 , 2009

18 Lieber CS, "Metabolism of alcohol" Clin Liver Dis 9 (1) : 1 ~ 35 , 2005

19 Lee WC, "Liver internal medicine" Oriental Medicine Research Press : 197 ~ , 1997

20 Palinski W, "Immune responses to oxidative neoepitopes on LDL and phospholipids modulate the development of atherosclerosis" J Intern Med 247 (3) : 371 ~ 380 , 2000

21 Corns CM, "Herbal remedies and clinical biochemistry" Ann Clin Biochem 40 (Pt 5) : 489 ~ 507 , 2003

22 심상인, "Hepatopotective Effects of Black Rice on Superoxide Anion Radicals in HepG2 Cells" Food Science and Biotechnology 15 (6) : 993 ~ 996 , 2006

23 Tapsell LC, "Health benefits of herbs and spices: the past, the present, the future" Med J Aust 185 (4 Suppl) : S4 ~ S24 , 2006

24 Zimmerman HJ, "Enzymes in hepatic disease In : Goodly EE (Ed), Diagnostic Enzymology" Lea and Febiger Publisher : 1 ~ 38 , 1970

25 Cohen G, "Elevation in blood acetaldehyde by pargyline during ethanol administration" Biochem Pharmacol 24 (2) : 313 ~ 316 , 1975

26 Kim JJ, "Eastern liver internal medicine" Jipmoondang Press : 27 ~ 36 , 1986

27 Huhjoon, "Donguibogam" Yeogang Press : 1680 ~ , 2003

28 Kim CM, "Complete translation chinese medicine dictionary" Jengdam Press : 1482 ~ 1486 , 1998

29 Lee GN, "Clinical Pathology Files" Medical Culture Publishers : 122 ~ , 1993

30 Go MS, "Clinical Application of Biochemical Liver Function Test" Dongguk J Med 15 (2) : 48 ~ 59 , 2008

31 Chenguiqian, "Bencaogangmutongshi" Xueyuan Press : 1449 ~ 1450 , 1992

32 Willcox JK, "Antioxidants and prevention of chronic disease" Crit Rev Food Sci Nutr 44 (4) : 275 ~ 295 , 2004

33 McDonough KH, "Antioxidant nutrients and alcohol" Toxicology 189 (1-2) : 89 ~ 97 , 2003

34 Berger TM, "Antioxidant activity of vitamin C in iron-overloaded human plasma" J Biol Chem 272 (25) : 15656 ~ 15660 , 1997

35 Kim YC, "An experimental research of the efficacy of Gamidaegumeumja" Kyunghee Univ Oriental Med J 16 : 7 ~ 29 , 1993

36 Willner IR, "Alcohol and the liver" Curr Opin Gastroenterol 21 (3) : 323 ~ 330 , 2005

37 Nordmann R, "Alcohol and antioxidant systems" Alcohol Alcohol 29 (5) : 513 ~ 522 , 1994