This study performed to investigate bioavailability in vitro from agriculture by-products. To simulate the enteric conditions, in vitro models for digestion and absorption were used. In vitro digestion model was divided into gastric phase and intestin...
This study performed to investigate bioavailability in vitro from agriculture by-products. To simulate the enteric conditions, in vitro models for digestion and absorption were used. In vitro digestion model was divided into gastric phase and intestinal phase. After digestion, samples were treated on the completely differentiated Caco-2 cell monolayer. To determine the transport of fat-soluble vitamin from foods to intestinal cells, absorption rate of fat-soluble vitamin through the intestinal epithelial cell was tested for extracted raw sample, aqueous fraction of digested sample and absorbed fraction through the Caco-2 cell were analyzed by HPLC. The green tea by-product sample showed higher vitamin E content than the other samples, whereas bioavailability was higher in the plum by-product samples better than the other samples. These results may provide useful information for the application of agriculture by-product sources on the functional food and animal feed.