Topical Anti-Inflammatory Activities of Vitis rotundifolia (Muscadine Grape) Extracts in the Tetradecanoylphorbol Acetate Model of Ear Inflammation

다국어 초록 (Multilingual Abstract)

The ability of muscadine grape skin, seed, or combined skin and seed extracts to inhibit mouse ear inflam-mation, edema, and polymorphonuclear leukocyte infiltration was tested following topical application of 12-O-tetrade-canoylphorbol 13-acetate (TPA). Ethanolic extracts of skins, seeds, or a combination of these from purple (Ison) cultivarswere applied to both ears of female Swiss mice 30 minutes after TPA (2 .g per ear) administration. Control mice were treatedwith indomethacin or 50% ethanol vehicle 30 minutes after TPA. Ear thickness was measured before TPA and at 4 and 24marker of edema. Myeloperoxidase (MPO) (EC 1.11.1.7) activity was measured in each ear punch biopsy as an index of neu-trophil infiltration. Extracts of muscadine skin, seed, and combination treatments significantly reduced ear edema, ear biopsyweight, and MPO activity compared to TPA vehicle control. There was no significant difference in anti-inflammatory activ-ity of the skin and seed extracts. However, an additive effect was observed with the combination treatment that was statisti-cally similar to the anti-inflammatory activity of indomethacin treatment. It can be concluded that muscadine skin, seed, andcombination skin/seed extracts exhibit significant topical anti-inflammatory properties.

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