Anti-inflammatory and Anti-tumorigenic Effects of Açai Berry in Helicobacter felis-infected mice

다국어 초록 (Multilingual Abstract)

Background: The aim of this study was to evaluate the anti-inflammatory and anti-tumorigenic effect of açai berry after chronic Helicobacter felis colonization in the stomachs of C57BL/6 mice.
Methods: A total of 57 four-week-old female C57BL/6 mice (18 control mice and 39 experimental mice) were used. The mice were administered orogastrically with vehicle only or vehicle containing H. felis, 5 times every other day. After inoculation of H. felis, mice were fed either a standard or an açai-containing diet and then sacrificed at 4, 24, and 52 weeks. The infection status and degree of inflammation were determined by culture and histopathology. The level of gastric mucosal myeloperoxidase (MPO), TNF-α, and interleukin-1β (IL-1β) were measured by ELISA.
Results: At 24 weeks after inoculation, mucosal atrophy and mucous metaplasia appeared in all infected mice. At 52 weeks after inoculation, dysplastic change was noted in 10%, 25%, and 50% of mice in the H. felis-control, H. felis-açai 5%, and H. felis-açai 10% groups, respectively. The neutrophil, monocyte, atrophy, and metaplasia grades of infected mice showed no significant difference among the H. felis-infected groups. H. felis-infected mice fed with açai berry showed no significant difference compared with H. felis-infected control mice in gastric mucosal MPO, TNF-α, and IL-1β levels.
Conclusions: H. felis that colonized the stomachs of C57BL/6 mice provoked inflammation, and induced mucosal atrophy, metaplasia, and dysplasia. However, açai berry did not effectively prohibit the gastric carcinogenesis which was induced by chronic H. felis infection.

참고문헌 (Reference)

1 Xie C, "The açaí flavonoid velutin is a potent anti-inflammatory agent:blockade of LPS-mediated TNF-α and IL-6 production through inhibiting NF-κB activation and MAPK pathway" 23 : 1184-1191, 2012

2 Shmuely H, "Susceptibility of Helicobacter pylori isolates to the antiadhesion activity of a high-molecular-weight constituent of cranberry" 50 : 231-235, 2004

3 Lim SH, "Prevalence and risk factors of Helicobacter pylori infection in Korea: nationwide multicenter study over 13 years" 13 : 104-, 2013

4 Schauss AG, "Phytochemical and nutrient composition of the freeze-dried amazonian palm berry, Euterpe oleraceae mart. (acai)" 54 : 8598-8603, 2006

5 National Research Council, "Nutrient Requirements of Laboratory Animals" National Academy Press 11-79, 1955

6 Stoner GD, "Multiple berry types prevent N-nitrosomethylbenzylamine-induced esophageal cancer in rats" 27 : 1138-1145, 2010

7 Hayakawa Y, "Mouse models of gastric cancer" 5 : 92-130, 2013

8 Gotteland M, "Modulation of Helicobacter pylori colonization with cranberry juice and Lactobacillus johnsonii La1 in children" 24 : 421-426, 2008

9 Lee A, "Isolation of a spiral-shaped bacterium from the cat stomach" 56 : 2843-2850, 1988

10 Chatterjee A, "Inhibition of Helicobacter pylori in vitro by various berry extracts, with enhanced susceptibility to clarithromycin" 265 : 19-26, 2004

11 Chen GG, "Induction of apoptosis in glioma cells by molecules released from activated macrophages" 57 : 179-186, 2002

12 이주엽, "Histologic Findings and Inflammatory Reactions After Long-term Colonization of Helicobacter felis in C57BL/6 Mice" 대한암예방학회 19 (19): 224-230, 2014

13 Fox JG, "Germ-line p53-targeted disruption inhibits helicobacter-induced premalignant lesions and invasive gastric carcinoma through down-regulation of Th1 proinflammatory responses" 62 : 696-702, 2002

14 Stoner GD, "Foodstuffs for preventing cancer: the preclinical and clinical development of berries" 2 : 187-194, 2009

15 Kang J, "Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and anti-inflammatory activities" 128 : 152-157, 2011

16 Zhang L, "Efficacy of cranberry juice on Helicobacter pylori infection: a double-blind, randomized placebo-controlled trial" 10 : 139-145, 2005

17 Moura RS, "Effects of Euterpe oleracea Mart. (AÇAÍ) extract in acute lung inflammation induced by cigarette smoke in the mouse" 19 : 262-269, 2012

18 McColl KE, "Clinical practice. Helicobacter pylori infection" 362 : 1597-1604, 2010

19 정규원, "Cancer Statistics in Korea: Incidence, Mortality, Survival, and Prevalence in 2012" 대한암학회 47 (47): 127-141, 2015

20 Fragoso MF, "Açai (Euterpe oleracea Mart.) feeding attenuates dimethylhydrazine-induced rat colon carcinogenesis" 58 : 68-76, 2013

21 Folmer F, "Anticancer effects of bioactive berry compounds" 13 : 295-322, 2014

22 Lee A, "A standardized mouse model of Helicobacter pylori infection:introducing the Sydney strain" 112 : 1386-1397, 1997