First Record of Three Uronychia Species (Ciliophora: Spirotrichea: Euplotida) from Korea

다국어 초록 (Multilingual Abstract)

Three morphospecies of the genus Uronychia, i.e. U. setigera Calkins, 1902, U. binucleata Young, 1922, and U. multicirrus Song, 1997, were collected from the coastal waters of Gumjin-ri on the East Sea and the public waterfront of Incheon on the Yellow Sea in Korea, respectively. These species are described based on live observation, protargol impregnation, silver nitrate impregnation, and their morphometrics. Diagnostic keys for these species are also provided. In addition, their small subunit ribosomal DNA sequences were compared with previously known sequences of Uronychia species. Diagnostics of three Uronychia species are as follows:U. setigera: 50-80 μm long in vivo, oval-shaped, 2 macronuclear nodules (Ma), 1 spur on the left margin, 11adoral membranelles (AM) 1, 4 AM2 , 1 buccal cirrus (BC), 4 frontal cirri (FC), 3 left marginal cirri (LMC), 2ventral cirri (VC), 5 transverse cirri (TC), 3 caudal cirri (CC), 6 dorsal kineties (DK), and approximately 23cilia in the leftmost kinety. U. binucleata: 70-110 μm long in vivo, oval to slightly rectangular shaped, 2 Ma, 1micronucleus (Mi), 2 spurs on the posterior region, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, 2 VC, 5 TC, 3 CC,6 DK, and approximately 37 cilia in the leftmost kinety. U. multicirrus: 140-200 μm long in vivo, oval to slightly rectangular shaped, ca. 7 Ma, 1 Mi, 11 AM1, 4 AM2, 1 BC, 4 FC, 3 LMC, approximately 8 VC, 5 TC, 3 CC,and 6 DK. This study presents the first record of this genus in Korea.

참고문헌 (Reference)

1 Claparède E, "Études sur les infusoires et les rhizopodes" 5 : 1-260, 1858

2 Wallengren J, "Zur Kenntnis der vergleichenden Morphologie der hypotrichen Infusorien" 26 : 1-31, 1900

3 Kahl A, "Urtiere oder Protozoa. I: Wimpertiere oder Ciliata (Infusoria), 3" 25 : 399-650, 1932

4 Jiang J, "Two new marine ciliates, Euplotes sinicus sp. nov. and Euplotes parabalteatus sp. nov., and a new small subunit rRNA gene sequence of Euplotes rariseta (Ciliophora, Spirotrichea, Euplotida)" 60 : 1241-1251, 2010

5 Lynn DH, "The ciliated protozoa: characterization, classification, and guide to the literature" Springer 1-605, 2008

6 Corliss JO, "The ciliated protozoa" Pergamon Press 1-455, 1979

7 Thompson JD, "The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools" 24 : 4876-4882, 1997

8 Foissner W, "Taxonomie und Ökologie einiger Ciliaten (Protozoa, Ciliophora) des Saprobiensystems. I: Genera Litonotus, Amphileptus, Opisthodon" 119 : 193-208, 1984

9 Pierantoni N, "Su alcuni Euplotidae del Golfo di Napoli" Bollettino della Societã di Naturalisti in Napoli 53-64, 1909

10 Bullington WE, "Some ciliates from Tortugas" 32 : 179-221, 1940

11 Song W, "Redefinition of the marine ciliate genus, Uronychia. In: Progress in protozoology (Eds., Song W, Xu K, Shi X, Hu X, Lei Y, Wei J, Chen Z, Shi X, Wang M)" Qingdao Ocean University Press 177-187, 1999

12 Taylor CV, "Protoplasmic reorganization of Uronychia uncinata sp. nov. during binary fission and regeneration" 1 : 1-25, 1928

13 Se-Joo Kim, "Optimization of DNA Extraction from a Single Living Ciliate for Stable and Repetitive PCR Amplification" 한국통합생물학회 13 (13): 351-356, 2009

14 Song W, "On the morphology and infraciliature of a new marine hypotrichous ciliate, Uronychia multicirrus sp. n. (Ciliophora, Hypotrichida)" 36 : 279-285, 1997

15 Song W, "Morphological investigations on some free living ciliates (Protozoa, Ciliophora) from China Sea with description of a new hypotrichous genus, Hemigastrostyla nov. gen" 148 : 413-444, 1997

16 Tamura K, "MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0" 24 : 1596-1599, 2007

17 Song W, "Free-living ciliates in the Bohai and Yellow Seas, China" Science Press 1-518, 2009

18 Shen Z, "Description of the rare marine ciliate, Uronychia multicirrus song, 1997 (Ciliophora; Euplotida) based on morphology, morphogenesis and SS rRNA gene sequence" 56 : 296-304, 2009

19 Song W, "Considerations on the systematic position of Uronychia and related euplotids based on the data of ontogeny and 18S rRNA gene sequence analyses, with morphogenetic redescription of Uronychia setigera Calkins, 1902 (Ciliophora: Euplotida)" 43 : 313-328, 2004

20 Fenchel T, "Ciliates from Scandinavian molluscs" 2 : 71-174, 1965

21 Hall TA, "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT" 95-98, 1999

22 Buddenbrock W, "Beobachtungen über einige neue oder wenig bekannte marine Infusorien" 41 : 341-364, 1920

23 Foissner W, "Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa" 27 : 313-330, 1991

24 Müller OF, "Animalcula infusoria fluviatilia et marina" N. Mölleri, Hauniae 1-367, 1786

25 Xu H, "An approach to analyses of periphytic ciliate communities for monitoring water quality using a modified artificial substrate in Korean coastal waters" Cambridge University Press 89 (89): 669-679, 2009

26 Curds CR, "A review of the Euplotidae (Hypotrichida, Ciliophora)" 28 : 1-63, 1983

27 Young DB, "A contribution to the morphology and physiology of the genus Uronychia" 36 : 353-395, 1922