Activation of C2H2-type Zinc Finger Genes Induces Dwarfism in Arabidopsis thaliana

다국어 초록 (Multilingual Abstract)

Zinc finger proteins compose one of the largesttranscription factor families in plants, but only a handful havebeen functionally specified in plant growth. In this study, a semidominantmutant, Arabidopsis thaliana dwarf1 (Atdwa1), wasdiscovered from activation tagging screening using the SKI015 TDNAvector. The Atdwa1 mutant displayed severe dwarfism andloss of apical dominance, as well as other pleiotropic defects, suchas earlier flowering, fewer leaves, and shorter sliliques than thoseof the wild-type plant. Thermal asymmetric interlaced- polymerasechain reaction analysis revealed that activated T-DNA wasinserted into the At5g54330 gene, disrupting its coding sequence.
We also demonstrated that At5g54330 expression was nullified bythe insertion, whereas expression of its downstream genes,At5g54340, At5g54350, and At5g54360, were activated. Adatabase search revealed that all three downstream genes encodeproteins related to C2H2-type zinc finger proteins. Overexpressionof each gene resulted in dwarfism similar to that of Atdwa1. Thesefindings indicate that Atdwa1 mutant was caused by synergisticmanifestation of concomitant activation of At5g54340 toAt5g54360, and that the zinc finger proteins may play a role inregulation of plant growth and development.

참고문헌 (Reference)

1 Magome H, "dwarf and delayed-flowering 1, a novel Arabidopsis mutant deficient in gibberellin biosynthesis because of overexpression of a putative AP2 transcription factor" 37 : 720-729, 2004

2 Takatsuji H, "Zinc-finger transcription factors in plants" 54 : 582-596, 1998

3 Takatsuji H, "Zinc-finger proteins: the classical zinc finger emerges in contemporary science" 39 : 1073-1078, 1999

4 Mackay JP, "Zinc fingers are sticking together" 23 : 1-4, 1998

5 Laity JH, "Zinc finger proteins new insights into structural and functional diversity" 11 : 39-46, 2001

6 Liu L, "Transcription factors and their genes in higher plants: Functional domains, evolution and regulation" 262 : 247-257, 1999

7 Iuchi S, "Three classes of C2H2 zinc finger proteins" 58 : 625-635, 2001

8 Ciftci-Yilmaz S, "The zinc finger network of plants" 65 : 1150-1160, 2008

9 Ephritikhine G, "The sax1 dwarf mutant of Arabidopsis thaliana shows altered sensitivity of growth responses to abscisic acid, auxin, gibberellins and ethylene and is partially rescued by exogenous brassinosteroid" 18 : 303-314, 1999

10 Bowman JL, "SUPERMAN, a regulator of floral homeotic genes in Arabidopsis" 114 : 599-615, 1992

11 Sakai H, "Role of SUPERMAN in maintaining Arabidopsis floral whorl boundaries" 378 : 199-202, 1995

12 Klug A, "Protein motifs 5: zinc fingers" 9 : 597-604, 1995

13 Gourcilleau D, "Phylogenetic Study of Plant Q-type C2H2 Zinc Finger Proteins and Expression Analysis of Poplar Genes in Response to Osmotic, Cold and Mechanical Stresses" 18 : 77-92, 2011

14 Sun Y, "Overexpression of the Arabidopsis gene UPRIGHT ROSETTE reveals a homeostatic control for indole-3-acetic acid" 153 : 1311-1320, 2010

15 Wang M, "Overexpression of a putative Arabidopsis BAHD acyltransferase causes dwarfism that can be rescued by brassinosteroid" 63 : 5787-5801, 2012

16 Clough SJ, "Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana" 16 : 735-743, 1998

17 Tague BW, "Expression analysis of an Arabidopsis C2H2 zinc finger protein gene" 32 : 785-796, 1997

18 Dinkins R, "Ectopic expression of an Arabidopsis single zinc finger gene in tobacco results in dwarf plants" 43 : 743-750, 2002

19 Englbrecht CC, "Conservation, diversification and expansion of C2H2 zinc finger proteins in the Arabidopsis thaliana genome" 5 : 39-, 2004

20 Guo YL, "Characterization of the uro mutant suggest that the URO gene is involved in the auxin action in Arabidopsis" 46 : 846-853, 2004

21 Riechmann JL, "Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes" 290 : 2105-2110, 2000

22 Weigel D, "Activation Tagging in Arabidopsis" 122 : 1003-1014, 2000