α‐l‐Arabinofuranosidases are important in the degradation of plant polysaccharides and are used in several industrial processes. Although the use of filamentous fungi for the production of α‐l‐arabinofuranosidases is widely reported, there a...
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https://www.riss.kr/link?id=O116606163
Liwei Gao ; Shiying Li ; Yanning Xu ; Chengqiang Xia ; Jiadi Xu ; Jun Liu ; Yuqi Qin ; Xin Song ; Guodong Liu ; Yinbo Qu
2019년
-
1860-6768
1860-7314
SCOPUS;SCIE
학술저널
n/a-n/a [※수록면이 p5 이하이면, Review, Columns, Editor's Note, Abstract 등일 경우가 있습니다.]
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
α‐l‐Arabinofuranosidases are important in the degradation of plant polysaccharides and are used in several industrial processes. Although the use of filamentous fungi for the production of α‐l‐arabinofuranosidases is widely reported, there a...
α‐l‐Arabinofuranosidases are important in the degradation of plant polysaccharides and are used in several industrial processes. Although the use of filamentous fungi for the production of α‐l‐arabinofuranosidases is widely reported, there are few reports on strain engineering for enhanced production of these enzymes by fungi. In this study, the function of transcription factor AraR in l‐arabinose release and catabolism by the fungus Penicillium oxalicum (P. oxalicum) is investigated. Also, a mutant of AraR, AraRA731V, is constructed to improve the production of α‐l‐arabinofuranosidases on the basis of the sequence homology between AraR and the xylanolytic gene activator XlnR. The AraRA731V‐overexpressing strain can synthesize α‐l‐arabinofuranosidase in the absence of an inducer and shows a 54.1‐fold increase in α‐l‐arabinofuranosidase production and a 7.4‐fold increase in α‐galactosidase production in the medium containing wheat bran. Determination of the transcript abundances of lignocellulolytic enzyme genes reveals significant upregulation of multiple α‐l‐arabinofuranosidase genes and downregulation of some cellulolytic and xylanolytic enzyme genes in the engineered strain relative to its parent. Taken together, the results suggest the conserved regulatory function of AraR in the family Trichocomaceae and provide a strategy for engineering fungal strains for enhanced α‐
l‐arabinofuranosidase production.
α‐l‐Arabinofuranosidases are important in plant cell‐wall degradation and are used in many industries. In this study, it is shown that a point mutation of transcription factor AraR could increase α‐l‐arabinofuranosidase production by 54.1‐fold in the fungus Penicillium oxalicum.
Editorial Board: Biotechnology Journal 7/2019
Cover Picture: Biotechnology Journal 7/2019