점망둑(Chasmichthys dolichognathus)의 최종성숙(GVBD)과 배란 유도에 미치는 Prostaglandins의 영향 = Effect of Prostaglandins on in vitro Oocyte Final Maturation (GVBD) and Ovulation in the Longchin Goby Chasmichthys dolichognathus

다국어 초록 (Multilingual Abstract)

Perhaps the most common type of reproductive dysfunction in captive fish is failure of females to undergo final oocyte maturation and thus to ovulate and spawn. The success of aquaculture could therefore be improved by developing techniques to enhance natural spawning, artificial maturation, and/or to induce ovulation in farmed fish. This study aimed to investigate the effects of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2α) on in vitro oocyte maturation (germinal vesicle breakdown, GVBD) and ovulation in the marine fish Chasmichthys dolichognathus.
Post-vitellogenic follicles (0.80-0.94 mm diameter oocytes) were incubated with PGE2 or PGF2α at concentrations of 5, 50, or 500 ng/mL for 24 hours. A significant increase in GVBD was seen in 0.84 mm and 0.94 mm oocytes incubated with 50 ng/mL PGE2 compared with the control. There was no significant increase in GVBD in any of the other experimental conditions (5 or 500 ng/mL PGE2 or 5, 50, or 500 ng/mL PGF2α). Neither of the prostaglandins induced ovulation at the concentrations tested. These results suggest that GVBD was induced by incubation with 50 ng/mL PGE2.

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