등열량 고지방식과 지구성 운동트레이닝이 흰쥐의 골격근 내 미토콘드리아 생합성과 지구성운동 능력에 미치는 영향 = The Effects of Isocaloric High Fat Diet and Endurance Exercise Training on mitochondrial biogenesis and Endurance Exercise Capacity of male Wistar rats

국문 초록 (Abstract)

본 연구의 목적은 등열량 고지방식과 지구성 운동이 미토콘드리아 생합성과 지구성 운동능력에 미치는 영향을 규명하는 것이다. 4주령의 male Wistar rat 60마리를 대상으로 4집단(Chow, HF-총열량의 50%를 지방으로 공급, Ex-트레드밀 달리기, 경사도 8%, 23 m/min, 120분/일, 5일/주, FEx)으로 무선 배정한 다음 총 4주간의 처치기간을 거쳐 지구성 운동능력과 골격근 내 변화를 관찰하였다. 연구결과 4주간의 등열량 고지방식 또는 중강도의 지구성 운동은 체중과 체지방량에 유의한 영향을 미치지 않았고, 등열량 고지방식은 골격근 내 포도당 이동률을 감소시키지 않았다. 또한 등열량 고지방식과 지구성운동 복합처치는 골격근 내 전자전달계 단백질 수준을 증가시키고, 근글리코겐 함량이 운동집단에 비해 50% 감소된 수준에서도 지구성 운동능력은 동일한 수준으로 나타났다. 이러한 결과를 통해 4주간의 등열량 고지방식은 골격근 내 인슐린 저항성을 발생시키지 않으며, 지구성운동과 복합처치 할 경우 미토콘드리아 생합성을 증가시키고 지구성 운동능력을 높이는 것으로 생각된다.

다국어 초록 (Multilingual Abstract)

The purpose of this study was to examine the effects of isocaloric high fat diet and endurance exercise training on mitochondrial biogenesis, endurance exercise capacity. The rats were randomly assigned into 4 groups; Chow, HF(50% of calories from fat), Ex(treadmill running, slop 8%, 23 m/min, 120 min/day, 5 d/week), FEx. 4-week of high fat diet and endurance exercise training did not change body weight and body fat. Further the level of insulin-stimulated glucose transport rate in soleus muscle was not affected by isocaloric high fat diet. Although either isocaloric high fat diet or endurance exercise alone did not change on mitochondrial biogenesis marker, isocaloric high fat diet with endurance exercise increase level of mitochondrial biogenesis marker. Despite muscle glycogen contents were significantly decreased in FEx, endurance exercise capacity was not decreased. With these results, 4-week isocaloric high fat did not cause insulin resistance in skeletal muscle, and when combined with endurance exercise training increases mitochondrial biogenesis and endurance exercise capacity.

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