Induction of Apoptosis in HT-29 Colon Cancer Cells by Phloretin

다국어 초록 (Multilingual Abstract)

Phloretin, which is present in apples and pears, has been found to inhibit the growth of several cancer cellsand induce apoptosis of B16 melanoma and HL60 human leukemia cells. The present study examined whether and howphloretin induces apoptosis of HT-29 human colon cancer cells. Phloretin (0100 .mol/L) substantially decreased viable cellthat phloretin increased the protein levels of Bax but had no effect on Bcl-2. In addition, phloretin induced cleavage of cas-pase-8, -9, -7, and -3 and poly(ADP-ribose) polymerase. Furthermore, phloretin increased the levels of cytochrome c andSmac/Diablo in the cytosol. The present results indicate that phloretin inhibits HT-29 cell growth by inducing apoptosis, whichmay be mediated through changes in mitochondrial membrane permeability and activation of the caspase pathways.

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