한국산 겨우살이 추출물 M11C(렉틴 구성물질)가단구세포의 TNF-α 유전자 발현유도 및 분비에 비치는 효과

다국어 초록 (Multilingual Abstract)

It is well-known that Korean mistletoe (Viscum album) extract has an immune activity and anticancer effect.
In this study, Korean mistletoe extract, M11C (non-lectin components), was used to examine whether this extract might activate
human peripheral monocyte to produce tumor necrosis factor-α (TNF-α). To examine the effect of M11C on the production
of TNF-α from monocyte, the monocyte were stimulated by the M11C, and then collected the supernatant (M11C
stimulated monocyte-conditioned media; MCM). MCM was treated to the TNF-α sensitive L929 cells, and then L929 cytotoxicity
was measured by means of MTT. MCM had cytotoxic effect on L929. And the cytotoxic effect of MCM on L929 was
almost abolished by anti-TNF-α antibody. These data indicated that MCM contained TNF-α, suggesting the TNF-α generation
from M11C-stimulated monocyte. This suggestion was confirmed from the data that TNF-α was highly detected in
MCM by immunoblotting technique. M11C effect on TNF-α production from monocyte was in the dose and stimulating
time dependent manners. Also the effect of M11C on the expression of TNF-α mRNA from monocyte was shown in the dose
and stimulating time dependent manners. As a result, Korean mistletoe extract, M11C, could be used for an immunostimulator.

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