S-allele Specific PCR 분석에 의한 사과와 꽃사과 품종의 자가불화합성 유전자형 동정

국문 초록 (Abstract)

사과는 배우체형 자가불화합성을 나타내는데 이는 S-locus의 복대립유전자에 의해 조절된다. 본 연구는 S-allele specific PCR분석을 통해 신품종을 포함한 24종의 사과 주요 재배품종과 7종의 꽃사과 품종의 자가불화합성 유전자형(S-genotype)을 결정하고자 수행하였다. 31종의 재배품종과 꽃사과 품종
을 23종의 S-allele specific primer을 이용하여 분석한 결과 12개의 S-allele (S1, S2, S3, S5, S7, S9, S10, S16, S21, S23, S26, S29)이 동정되었다. 그 중에서 24종의 재배품종에는 S1(41.7%), S3(58.3%), S7(29.2%), S9(54.2%)의 S-allele이 흔히 존재하는 것으로 확인되었다. 국내육성 신품종인 ‘아리수’와 ‘황옥’의 S-genotype은 각각 S3S7과 S3S9으로 동정되었다. 본 실험에서 얻은 S-genotype 정보는 안정적인 사과 과실생산에 적합한 수분수 선발과 육종프로그램에서 교배조합 작성에 유용
하게 활용될 것이다.

다국어 초록 (Multilingual Abstract)

Apple (Malus ☓ domestica Borkh.) has gametophytic self-incompatibility (S) controlled by the multi-allelic S-locus. In the present study, S-genotypes of 24 apple cultivars including newly released Korean cultivars and seven crabapple cultivars were identified using S-allele specific polymerase chain reaction analysis. Twelve different S-alleles (S1, S2, S3, S5, S7, S9, S10, S16, S21, S23, S26, and S29) from 31 apple and crabapple cultivars were identified using 23 S-allele specific primers. Among them, S1 (41.7%), S3 (58.3%), S7 (29.2%), and S9 (54.2%) S-alleles were found to be common in 24 apple cultivars. The newly released Korean cultivars ‘Arisoo’ and ‘Hwangok’ were genetyped as S3S7 and S3S9, respectively. S-genotypes information obtained from the present study will be useful to select proper pollinzers for stable production of apple fruit and to design cross of breeding programs.

목차 (Table of Contents)

• Abstract
• 서 언
• 재료 및 방법
• 시험재료 및 Genomic DNA 추출
• S-allele specific primer를 이용한 PCR분석
• 결과 및 고찰
• 적 요
• REFERENCES

참고문헌 (Reference)

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